Evidence for Increased Expression of SIRT3 Associated with Hyperandrogenism in Granulosa Cells of Non-Obese PCOS Patients

作者全名："Zhang, Shen; Deng, Wenli; Liu, Qiongyou; Yang, Wei"

作者地址："[Zhang, Shen] Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Reprod Med Ctr, Chongqing 400010, Peoples R China; [Zhang, Shen] Wenzhou Med Univ, Affiliated Hosp 1, Reprod Med Ctr, Wenzhou 325000, Zhejiang, Peoples R China; [Deng, Wenli] Chongqing Med Univ, Affiliated Hosp 1, Chongqing Key Lab Ophthalmol, Chongqing 400016, Peoples R China; [Deng, Wenli] Chongqing Eye Inst, Chongqing 400016, Peoples R China; [Liu, Qiongyou] Zunyi Med Univ, Sch Basic Med Sci, Zunyi 563003, Guizhou, Peoples R China; [Yang, Wei] Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Inst Biomed Engn, Shenzhen 518020, Guangdong, Peoples R China; [Yang, Wei] Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China"

通信作者："Zhang, S (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Reprod Med Ctr, Chongqing 400010, Peoples R China.; Zhang, S (通讯作者)，Wenzhou Med Univ, Affiliated Hosp 1, Reprod Med Ctr, Wenzhou 325000, Zhejiang, Peoples R China."

来源：CLINICAL AND EXPERIMENTAL OBSTETRICS & GYNECOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000917832300021

JCR分区：Q4

影响因子：0.2

年份：2022

卷号：49

期号：11

开始页：　

结束页：　

文献类型：Article

关键词：SIRT3; polycystic ovary syndrome; luteinized granulosa cells; hyperandrogenism; oxidative stress

摘要："Background: SIRT3 regulates the generation of reactive oxygen species (ROS) in human granulosa cells (GCs). Increased levels of oxidative stress may cause follicular dysplasia in GCs of polycystic ovary syndrome (PCOS) patients. However, expression and regulation of SIRT3 in GCs of PCOS patients have not yet been investigated. The present study is conducted to determine the correlation between SIRT3 and hyperandrogenism in luteinized GCs of PCOS patients. Methods: The mRNA and protein expression of SIRT3 were analysed in the luteinized GCs from the controls and non-obese PCOS patients. Dihydrotestosterone (DHT) was added to the primary cultured GCs to test the effects of androgen excess on intracellular ROS and SIRT3 expression. A DHT-induced PCOS murine model was used to confirm the effects in vivo. Results: In the matched case-control study including 32 pairs of the controls and non-obese PCOS patients, we showed that the expression of SIRT3 was increased in luteinized GCs of non-obese PCOS patients compared with normovulatory controls. Moreover, DHT induced oxidative stress and SIRT3 expression in human GCs, which was further confirmed in a murine PCOS model. Conclusions: These results indicated that the increased expression of SIRT3 was induced by hyperandrogenism in GCs of non-obese PCOS patients."

基金机构：Natural Science Foundation of Chongqing Municipality [cstc2021jcyjmsxmX0097]; Natural Science Foundation of Zhejiang Province [LQ19H040003]; Natural Science Foundation of Zunyi Medical University [F-585]

基金资助正文："This research was funded by the Natural Science Foundation of Chongqing Municipality (No. cstc2021jcyjmsxmX0097 to SZ), the Natural Science Foundation of Zhejiang Province (No. LQ19H040003 to SZ) and the Natural Science Foundation of Zunyi Medical University (No. F-585 to QL)."