A Stand-Alone Microfluidic Chip for Long-Term Cell Culture

作者全名："Feng, Yibo; Zeng, Yang; Fu, Jiahao; Che, Bingchen; Jing, Guangyin; Liu, Yonggang; Sun, Dan; Zhang, Ce"

作者地址："[Feng, Yibo; Zeng, Yang; Fu, Jiahao; Sun, Dan; Zhang, Ce] Northwest Univ, Inst Photon & Photon Technol, State Key Lab Photon Technol Western China Energy, 1, Xuefu Ave, Xian 710127, Peoples R China; [Che, Bingchen; Jing, Guangyin] Northwest Univ, Sch Phys, 1 Xuefu Ave, Xian 710127, Peoples R China; [Liu, Yonggang] Chongqing Med Univ, Lab Stem Cell & Tissue Engn, Chongqing 400016, Peoples R China; [Sun, Dan; Zhang, Ce] RongGuang Yun Biotechnol Co Ltd, Qin Han Innovat Ctr, G2018, Bldg C, Xianyang 712039, Peoples R China"

通信作者："Sun, D; Zhang, C (通讯作者)，Northwest Univ, Inst Photon & Photon Technol, State Key Lab Photon Technol Western China Energy, 1, Xuefu Ave, Xian 710127, Peoples R China.; Liu, YG (通讯作者)，Chongqing Med Univ, Lab Stem Cell & Tissue Engn, Chongqing 400016, Peoples R China.; Sun, D; Zhang, C (通讯作者)，RongGuang Yun Biotechnol Co Ltd, Qin Han Innovat Ctr, G2018, Bldg C, Xianyang 712039, Peoples R China."

来源：MICROMACHINES

ESI学科分类：ENGINEERING

WOS号：WOS:000918462500001

JCR分区：Q2

影响因子：3.4

年份：2023

卷号：14

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：cell culture; fluidic chip; ITO glass; in-field; live cell

摘要："Live-cell microscopy is crucial for biomedical studies and clinical tests. The technique is, however, limited to few laboratories due to its high cost and bulky size of the necessary culture equipment. In this study, we propose a portable microfluidic-cell-culture system, which is merely 15 cmx11 cmx9 cm in dimension, powered by a conventional alkali battery and costs less than USD 20. For long-term cell culture, a fresh culture medium exposed to 5% CO2 is programmed to be delivered to the culture chamber at defined time intervals. The 37? culture temperature is maintained by timely electrifying the ITO glass slide underneath the culture chamber. Our results demonstrate that 3T3 fibroblasts, HepG2 cells, MB-231 cells and tumor spheroids can be well-maintained for more than 48 h on top of the microscope stage and show physical characters (e.g., morphology and mobility) and growth rate on par with the commercial stage-top incubator and the widely adopted CO2 incubator. The proposed portable cell culture device is, therefore, suitable for simple live-cell studies in the lab and cell experiments in the field when samples cannot be shipped."

基金机构：National Natural Science Foundation of China [51927804]; China Scholarship Council [202106970013]; Natural Science Foundation of Shanxi Province [2021JM-301]

基金资助正文："This research was funded by the National Natural Science Foundation of China (51927804), China Scholarship Council (202106970013) and the Natural Science Foundation of Shanxi Province (2021JM-301)."