Transcriptome and pan-cancer system analysis identify PM2.5-induced stanniocalcin 2 as a potential prognostic and immunological biomarker for cancers

作者全名："Zhu, Dong; Liu, Jiliu; Wang, Junyi; Zhang, Lei; Jiang, Manling; Liu, Yao; Xiong, Ying; He, Xiang; Li, Guoping"

作者地址："[Zhu, Dong; Wang, Junyi; Zhang, Lei; Jiang, Manling; Li, Guoping] Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China; [Zhu, Dong; Liu, Jiliu; Wang, Junyi; Zhang, Lei; Jiang, Manling; Liu, Yao; He, Xiang; Li, Guoping] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Chengdu Inst Resp Hlth, Lab Allergy & Precis Med,Affiliated Hosp, Chengdu, Peoples R China; [Liu, Jiliu; Wang, Junyi; Zhang, Lei; Jiang, Manling; Liu, Yao; He, Xiang; Li, Guoping] ChongQing Med Univ, Chengdu Peoples Hosp 3, Dept Pulm & Crit Care Med, Affiliated Hosp,Branch Natl Clin Res,Ctr Resp Dis, Chengdu, Peoples R China; [Xiong, Ying] Sichuan Friendship Hosp, Dept Pulm & Crit Care Med, Chengdu, Peoples R China"

通信作者："Li, GP (通讯作者)，Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China.; He, X; Li, GP (通讯作者)，Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Chengdu Inst Resp Hlth, Lab Allergy & Precis Med,Affiliated Hosp, Chengdu, Peoples R China.; He, X; Li, GP (通讯作者)，ChongQing Med Univ, Chengdu Peoples Hosp 3, Dept Pulm & Crit Care Med, Affiliated Hosp,Branch Natl Clin Res,Ctr Resp Dis, Chengdu, Peoples R China.; Xiong, Y (通讯作者)，Sichuan Friendship Hosp, Dept Pulm & Crit Care Med, Chengdu, Peoples R China."

来源：FRONTIERS IN GENETICS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000918875000001

JCR分区：Q2

影响因子：3.7

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：PM2.5; prognosis; biomarker; cancer; stanniocalcin 2 (STC2)

摘要："Epidemiological studies have shown that air pollution and particulate matter (PM) are closely related to the occurrence of cancer. However, the potential prognostic and immunological biomarkers for air pollution related cancers are lacking. In this study, we proved PM2.5 exposure was correlated with lung cancer through transcriptome analysis. Importantly, we identified STC2 as a key gene regulated by PM2.5, whose expression in epithelial cells was significantly increased after PM2.5 treatment and validated by using RT-qPCR and immunofluorescence. Kaplan-Meier OS curves suggested that high STC2 expression positively correlated with a poor prognosis in lung cancer. Furthermore, we discovered that STC2 was associated with multiple cancers and pathways in cancer. Next, Pan-Cancer Expression Landscape of STC2 showed that STC2 exhibited inconsistent expression across 26 types of human cancer, lower in KIRP in cancer versus adjacent normal tissues, and significantly higher in another cancers. Cox regression results suggested that STC2 expression was positively or negatively associated with prognosis in different cancers. Moreover, STC2 expression was associated with clinical phenotypes including age, gender, stage and grade. Mutation features of STC2 were also analyzed, in which the highest alteration frequency of STC2 was presented in KIRC with amplification. Meanwhile, the effects of copy number variation (CNV) on STC2 expression were investigated across various tumor types, suggesting that STC2 expression was significantly correlated with CNV in tumors. Additionally, STC2 was closely related to tumor heterogeneity, tumor stemness and tumor immune microenvironment like immune cell infiltration. In the meantime, we analyzed methylation modifications and immunological correlation of STC2. The results demonstrated that STC2 expression positively correlated with most RNA methylation genes and immunomodulators across tumors. Taken together, the findings revealed that PM2.5-induced STC2 might be a potential prognostic and immunological biomarker for cancers related to air pollution."

基金机构："National Natural Science Foundation of China [81970026, 82000029, 82200079]; Natural Science Foundation of Sichuan [2022NSFCS1324]; Chengdu High-level Key Clinical Specialty Construction Project [ZX20201202020]; Chengdu Science and Technology Bureau [2021-YF09-00102-SN, 2020-YF05-00003-SN]; Sichuan Medical Association [Q21061]; Health Commission of Chengdu [2022193, 2022194]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (81970026, 82000029, and 82200079), Natural Science Foundation of Sichuan (2022NSFCS1324), Chengdu High-level Key Clinical Specialty Construction Project (ZX20201202020), Chengdu Science and Technology Bureau (2021-YF09-00102-SN and 2020-YF05-00003-SN), Sichuan Medical Association (Q21061), and the Health Commission of Chengdu (2022193 and 2022194)."