DUSP10 upregulation is a poor prognosticator and promotes cell proliferation and migration in glioma
作者全名："Zhou, Fang; Zeng, Lingfeng; Chen, Xi; Zhou, Fan; Zhang, Zhen; Yuan, Yixiao; Wang, Heping; Yao, Huayi; Tian, Jintao; Liu, Xujie; Zhao, Jinxi; Huang, Xiaobin; Pu, Jun; Cho, William C.; Cao, Jianxiong; Jiang, Xiulin"
作者地址："[Zhou, Fang] Hunan Univ Chinese Med, Changsha, Peoples R China; [Zhou, Fang; Zhang, Zhen] Hunan Acad Tradit Chinese Med, Affiliated Hosp, Dept Oncol, Changsha, Peoples R China; [Zeng, Lingfeng] Prince Wales Hosp, Carol & Richard Yu Peritoneal Dialysis Res Ctr, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China; [Zeng, Lingfeng] Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci LiHS, Fac Med, Shatin, Hong Kong, Peoples R China; [Chen, Xi; Wang, Heping; Yao, Huayi; Tian, Jintao; Liu, Xujie; Zhao, Jinxi; Huang, Xiaobin; Pu, Jun] Kunming Med Univ, Dept Neurosurg, Affiliated Hosp 2, Kunming, Peoples R China; [Zhou, Fan] Kunming Med Univ, Dept Hematol, Affiliated Hosp 2, Kunming, Yunnan, Peoples R China; [Yuan, Yixiao] Chongqing Med Univ, Affiliated Hosp 1, Key Lab Mol Oncol & Epigenet, Chongqing, Peoples R China; [Cho, William C.] Queen Elizabeth Hosp, Dept Clin Oncol, Hong Kong, Peoples R China; [Cao, Jianxiong] Hunan Univ Chinese Med, Dept Oncol, Hosp 1, Changsha, Peoples R China; [Jiang, Xiulin] Univ Chinese Acad Sci, Kunming Coll Life Sci, Beijing, Peoples R China"
通信作者："Cho, WC (通讯作者)，Queen Elizabeth Hosp, Dept Clin Oncol, Hong Kong, Peoples R China.; Cao, JX (通讯作者)，Hunan Univ Chinese Med, Dept Oncol, Hosp 1, Changsha, Peoples R China.; Jiang, XL (通讯作者)，Univ Chinese Acad Sci, Kunming Coll Life Sci, Beijing, Peoples R China."
来源：FRONTIERS IN ONCOLOGY
关键词：glioma; DUSP10; prognosticator; cell proliferation; cell migration
摘要："Dual-specificity phosphatase 10 (DUSP10) correlates with inflammation, cytokine secretion, cell proliferation, survival, and apoptosis. However, its role in glioma is unclear. Herein, we sought to examine the expression and the underlying carcinogenic mechanisms of DUSP10 action in glioma. DUSP10 expression in glioma was significantly higher than that in normal brain tissues. High DUSP10 expression indicated adverse clinical outcomes in glioma patients. Increased DUSP10 expression correlated significantly with clinical features in glioma. Univariate Cox analysis showed that high DUSP10 expression was a potential independent marker of poor prognosis in glioma. Furthermore, DUSP10 expression in glioma correlated negatively with its DNA methylation levels. DNA methylation level of DUSP10 also correlated negatively with poor prognosis in glioma. More importantly, DUSP10 expression correlated positively with the infiltration of B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cells in glioma. Gene set enrichment analysis (GSEA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis confirmed that DUSP10 participated in signaling pathways involved in focal adhesion, TNF cascade, Th17 cell differentiation, and NF-kappa B cascade. Finally, we uncovered that DUSP10 was dramatically upregulated in glioblastoma (GBM) cells and that the knockdown of DUSP10 inhibited glioma cell proliferation and migration. Our findings suggested that DUSP10 may serve as a potential prognostic biomarker in glioma."
基金机构："Yunnan Applied Basic Research Projects [2017FE467, 2018FE001]"
基金资助正文：This work was supported by the Yunnan Applied Basic Research Projects (2017FE467 and 2018FE001).