Study on differentially expressed genes between stage M and stage MS neuroblastoma

作者全名："Wu, Yuying; Zhang, Jun"

作者地址："[Wu, Yuying; Zhang, Jun] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Surg Oncol, Minist Educ,Key Lab Child Dev & Disorders,Children, Chongqing, Peoples R China"

通信作者："Zhang, J (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Surg Oncol, Minist Educ,Key Lab Child Dev & Disorders,Children, Chongqing, Peoples R China."

来源：FRONTIERS IN ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000920863300001

JCR分区：Q2

影响因子：4.7

年份：2023

卷号：12

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：neuroblastoma; stage MS; DEGs; immunohistochemistry; apoptosis; ferroptosis

摘要："ObjectiveTo search for the DEGs between stage MS NB and stage M NB and speculate the possible mechanism of spontaneous regression of stage MS NB. Materials and methodsThe NB datasets GSE49710 and GSE45547 in the GEO database were selected to screen the DEGs between children with NB stage MS vs. stage M, < 18 months. GO enrichment and KEGG pathway analysis of DEGs was performed using DAVID. The intersecting genes among DEGs and RCD-related genes were selected, and their survival roles and functions were assessed. We then used the collected clinical samples to validate the expression of these genes at the protein level using IHC methods and further analysis to explore their role. ResultsBIRC5, SLCO4A1, POPDC3, and HK2 were found to be downregulated in stage MS NB and related to apoptosis. BIRC5 and HK2 also participate in autophagy. The TF gene is upregulated in stage MS NB and related to ferroptosis. The above five genes are closely related to the survival of children with NB. And the expression levels of all five genes at the protein level were verified by IHC to be consistent with the results of the preliminary screening described above. ConclusionBIRC5, SLCO4A1, POPDC3, HK2 and TF are expected to become new important indicators to predict the prognosis of NB and can be used as the basis for further explored the benign prognosis and spontaneous regression mechanism of stage MS NB."

基金机构：Nature Science Foundation of Chongqing Municipality [cstc2017jcyjAX0139]

基金资助正文：All phases of this study were supported by a Nature Science Foundation of Chongqing Municipality grant (cstc2017jcyjAX0139).