Multiomics integration reveals the effect of Orexin A on glioblastoma
作者全名:"Yang, Sha; Huan, Renzheng; Yue, Jianhe; Guo, Jin; Deng, Mei; Wang, Liya; Peng, Shuo; Lin, Xin; Liu, Lin; Wang, Jia; Han, Guoqiang; Zha, Yan; Liu, Jian; Zhang, Jiqin; Tan, Ying"
作者地址:"[Yang, Sha; Guo, Jin; Liu, Jian] Guizhou Univ, Med Coll, Guiyang, Guizhou, Peoples R China; [Huan, Renzheng; Yue, Jianhe] Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 2, Chongqing, Peoples R China; [Deng, Mei; Wang, Liya; Peng, Shuo; Han, Guoqiang; Liu, Jian; Tan, Ying] Guizhou Prov Peoples Hosp, Dept Neurosurg, Guiyang, Peoples R China; [Lin, Xin; Zha, Yan] Guizhou Prov Peoples Hosp, Dept Nephrol, Guiyang, Peoples R China; [Liu, Lin] Guizhou Prov Peoples Hosp, Dept Resp & Crit Care Med, Guiyang, Peoples R China; [Wang, Jia] Chongqing Emergency Med Ctr, Dept Neurosurg, Chongqing, Peoples R China; [Zhang, Jiqin] Guizhou Prov Peoples Hosp, Dept Anesthesiol, Guiyang, Peoples R China"
通信作者:"Liu, J (通讯作者),Guizhou Univ, Med Coll, Guiyang, Guizhou, Peoples R China.; Liu, J; Tan, Y (通讯作者),Guizhou Prov Peoples Hosp, Dept Neurosurg, Guiyang, Peoples R China.; Zhang, JQ (通讯作者),Guizhou Prov Peoples Hosp, Dept Anesthesiol, Guiyang, Peoples R China."
来源:FRONTIERS IN PHARMACOLOGY
ESI学科分类:PHARMACOLOGY & TOXICOLOGY
WOS号:WOS:000925328400001
JCR分区:Q1
影响因子:4.4
年份:2023
卷号:14
期号:
开始页:
结束页:
文献类型:Article
关键词:glioblastoma multiforme; Orexin A; multi-omics; functional enrichment analyses; genes
摘要:"Objectives: This study involved a multi-omics analysis of glioblastoma (GBM) samples to elaborate the potential mechanism of drug treatment.Methods: The GBM cells treated with or without orexin A were acquired from sequencing analysis. Differentially expressed genes/proteins/metabolites (DEGs/ DEPs/ DEMs) were screened. Next, combination analyses were conducted to investigate the common pathways and correlations between the two groups. Lastly, transcriptome-proteome-metabolome association analysis was carried out to determine the common pathways, and the genes in these pathways were analyzed through Kaplan-Meier (K-M) survival analysis in public databases. Cell and animal experiments were performed to investigate the anti-glioma activity of orexin A.Results: A total of 1,527 DEGs, 52 DEPs, and 153 DEMs were found. Moreover, the combination analyses revealed that 6, 4, and 1 common pathways were present in the transcriptome-proteome, proteome-metabolome, and transcriptome-metabolome, respectively. Certain correlations were observed between the two data sets. Finally, 11 common pathways were discovered in association analysis, and 138 common genes were screened out in these common pathways. Six genes showed significant differences in terms of survival in both TCGA and CGGA. In addition, orexin A inhibited the proliferation, migration, and invasion of glioma in vitro and in vivo.Conclusion: Eleven common KEGG pathways with six common genes were found among different omics participations, revealing the underlying mechanisms in different omics and providing theoretical basis and reference for multi-omics research on drug treatment."
基金机构:"National Natural Science Foundation of China [81960454, 81960344, 82260533]; Guizhou Provincial Science and Technology Projects [(2020)1Z066]; Guizhou Provincial People's Hospital Doctor Foundation [(2018)06, (2018)03]; Guizhou Provincial People's Hospital National Science Foundation [GPPH-NSFC-2019-18, GPPH-NSFC-2019-09, GPPH-NSFC-D-2019-17]; National Science Foundation of Chongqing [cstc2020jcyj-msxmX0871]"
基金资助正文:"This work was supported by the National Natural Science Foundation of China (81960454, 81960344, 82260533), the Guizhou Provincial Science and Technology Projects ((2020)1Z066), Guizhou Provincial People's Hospital Doctor Foundation ((2018)06 and (2018)03), Guizhou Provincial People's Hospital National Science Foundation (GPPH-NSFC-2019-18, GPPH-NSFC-2019-09 and GPPH-NSFC-D-2019-17) and National Science Foundation of Chongqing (cstc2020jcyj-msxmX0871)."