Genetically engineered cell membrane-coated nanoparticles for antibacterial and immunoregulatory dual-function treatment of ligature-induced periodontitis
作者全名:"Deng, Yangjia; Ren, Mingxing; He, Ping; Liu, Fengyi; Wang, Xu; Zhou, Chongjing; Li, Yuzhou; Yang, Sheng"
作者地址:"[Deng, Yangjia; Ren, Mingxing; He, Ping; Liu, Fengyi; Wang, Xu; Zhou, Chongjing; Li, Yuzhou; Yang, Sheng] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Deng, Yangjia; Ren, Mingxing; He, Ping; Liu, Fengyi; Wang, Xu; Zhou, Chongjing; Li, Yuzhou; Yang, Sheng] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Deng, Yangjia; Ren, Mingxing; He, Ping; Liu, Fengyi; Wang, Xu; Zhou, Chongjing; Li, Yuzhou; Yang, Sheng] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"
通信作者:"Yang, S (通讯作者),Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China.; Yang, S (通讯作者),Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Yang, S (通讯作者),Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."
来源:FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ESI学科分类:Multidisciplinary
WOS号:WOS:000930375700001
JCR分区:Q1
影响因子:4.3
年份:2023
卷号:11
期号:
开始页:
结束页:
文献类型:Article
关键词:cell membrane coating; biomimetic nanoparticles; periodontitis; antibacterial therapy; immune regulation; TLR4
摘要:"Purpose: In order to overcome the problem that conventional pharmacological treatments of periodontitis cannot effectively synergizing antimicrobial and immunomodulation, inspired by the critical role of toll-like receptor 4 (TLR4) in bacterial recognition and immune activation, we demonstrated a combined antibacterial-immunoregulatory strategy based on biomimetic nanoparticles. Methods: Functioned cell membranes and silk fibroin nanoparticles (SNs) loaded with minocycline hydrochloride (Mino) were used to prepare a biomimetic nanoparticle (MSNCs). SNs and MSNCs were characterized by Scanning Electron Microscope, size, zeta potential, dispersion index. At the same time, SNs were characterized by cell counting kit-8 and real-time Polymerase Chain Reaction (RT-PCR). TLR4-expressing cell membranes were characterized by RT-PCR and western blot (WB). Cell membrane coating was characterized by Transmission Electron Microscope (TEM), the Bradford staining and WB. Then, Laser confocal, flow cytometry and agar plate coating were evaluated in vitro with antibacterial effects, RT-PCR was simultaneously evaluated with immunoregulatory effects. Finally, Anti-inflammatory treatment of MSNCs was evaluated in a ligature-induced periodontitis (LIP) mouse model. Results: Successfully prepared cell membranes overexpressing TLR4 and constructed MSNCs. In vitro studies had shown that MSNCs effectively targeted bacteria via TLR4 and acted as molecular decoys to competitively neutralize lipopolysaccharide (LPS) in the microenvironment as well as inhibit inflammatory activation of macrophages. In vivo, MSNCs effectively attenuated periodontal tissue inflammation and alveolar bone loss in a LIP mouse model. Conclusion: MSNCs have good targeted antibacterial and immunoregulatory effects, and provide a new and effective strategy for the treatment of periodontitis and have good potential for application in various types of pathogenic bacterial infections."
基金机构:"National Natural Science Foundation of China [82171010]; Natural Science Foundation of Chongqing [cstc2021jcyj-jqX0028]; Chongqing Science and Health Joint Project [2020GDRC015]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0079]"
基金资助正文:"This work was supported by the National Natural Science Foundation of China (Grant No. 82171010), the Natural Science Foundation of Chongqing (Grant No. cstc2021jcyj-jqX0028), Chongqing Science and Health Joint Project (Grant No. 2020GDRC015), Program for Youth Innovation in Future Medicine, Chongqing Medical University (No. W0079)."