Fast detection of beta(2) microglobulin in patient blood by a handhold centrifugal microfluidic device
作者全名："Ning, Ke; Chai, Huihui; Cui, Yanxia; Wu, Shiming; Chen, Feng; Li, Yuan; Qiu, Ping; Sun, Wei; Yu, Ling"
作者地址："[Ning, Ke; Chai, Huihui; Cui, Yanxia; Wu, Shiming; Chen, Feng; Yu, Ling] Southwest Univ, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Inst Clean Energy & Adv Mat,Sch Mat & Energy, Chongqing 400715, Peoples R China; [Li, Yuan] Chongqing Med Univ, Yongchuan Hosp, Yongchuan 402160, Peoples R China; [Qiu, Ping] Chengdu Med Coll, Affiliated Hosp 1, Chengdu 610500, Peoples R China; [Sun, Wei] Hainan Normal Univ, Coll Chem & Chem Engn, Haikou 571158, Hainan, Peoples R China"
通信作者："Yu, L (通讯作者)，Southwest Univ, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Inst Clean Energy & Adv Mat,Sch Mat & Energy, Chongqing 400715, Peoples R China.; Li, Y (通讯作者)，Chongqing Med Univ, Yongchuan Hosp, Yongchuan 402160, Peoples R China."
来源：SENSORS AND ACTUATORS B-CHEMICAL
关键词：Microfluidic chip; Centrifugal chip; Latex agglutination assay; beta(2) microglobulin; Point-of-care test
摘要："Elevating blood beta(2) microglobulins (beta(2) M) level is one of the indicators of diseases such as diabetes, kidney failure, and tumors. A centrifugal microfluidic chip was designed to conduct latex immunoagglutination assays for testing beta(2) M. A handhold centrifuge was assembled to drive solution mixing in the microfluidic chip. An office scanner recorded the binding of beta(2) M to the latex beads induced agglutination, reducing reliance on expensive scientific equipment and avoiding environmental light interference. The results revealed that the degree of beads agglutination was proportional to the grayscale value changes (Delta G). The beta(2) M testing can be accomplished using a 1.25 mu L blood sample, and the testing time is 10 min. The limit of detection is 0.0335 mg/L. The recovery rate calculated by the measured and spiked beta(2) M concentration ratio is 91.8-111.8%. The centrifugal chip was applied to test 50 patients' samples. The confusion matrix analyzed the rate of false positives, false negatives, true positives, and true negatives for the beta(2) M chip-based test, showing the sensitivity of the beta(2) M chip is 77.3%, the specificity is 82.1%, and the efficiency is 80%. The Area Under the Curve analysis shows that the cutoff value (Delta G = 1.011) of Delta G in predicting beta(2) M elevating has a sensitivity of 100% (95% CI: 92.87%-100%) with the specificity of 92% (95% CI:81.6-96.85%)."
基金机构："National Natural Science Foundation of China [31872753, 32171401]; Graduate Student's Research and Innovation Fund of Chongqing, China [CYS22206]; Innovation Platform for Academicians of Hainan Province [YSPTZX202126]"
基金资助正文："This work was financially supported by the National Natural Science Foundation of China (No. 31872753 and 32171401), Graduate Student's Research and Innovation Fund of Chongqing, China (CYS22206), and the specific research fund of The Innovation Platform for Academicians of Hainan Province (YSPTZX202126)."