Dynamic expression of Mage-D1 in rat dental germs and potential role in mineralization of ectomesenchymal stem cells

作者全名："Li, Meng; Yu, Xia; Luo, Yuting; Yuan, Hongyan; Zhang, Yixing; Wen, Xiujie; Zhou, Zhi"

作者地址："[Li, Meng; Luo, Yuting; Zhou, Zhi] Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn High, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Yu, Xia; Yuan, Hongyan; Zhang, Yixing; Wen, Xiujie] Southwest Med Univ, Hosp Stomatol, Dept Orthodont, Luzhou, Sichuan, Peoples R China"

通信作者："Zhou, Z (通讯作者)，Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn High, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Wen, XJ (通讯作者)，Southwest Med Univ, Hosp Stomatol, Dept Orthodont, Luzhou, Sichuan, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:000934089000036

JCR分区：Q1

影响因子：4.6

年份：2022

卷号：12

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Mage-D1 (MAGE family member D1) is involved in a variety of cell biological effects. Recent studies have shown that Mage-D1 is closely related to tooth development, but its specific regulatory mechanism is unclear. The purpose of this study was to investigate the expression pattern of Mage-D1 in rat dental germ development and its differential mineralization ability to ectomesenchymal stem cells (EMSCs), and to explore its potential mechanism. Results showed that the expression of Mage-D1 during rat dental germ development was temporally and spatially specific. Mage-D1 promotes the proliferation ability of EMSCs but inhibits their migration ability. Under induction by mineralized culture medium, Mage-D1 promotes osteogenesis and tooth-forming ability. Furthermore, the expression pattern of Mage-D1 at E19.5 d rat dental germ is similar to p75 neurotrophin receptor (p75NTR), distal-less homeobox 1 (Dlx1) and msh homeobox 1 (Msx1). In addition, Mage-D1 is binding to p75NTR, Dlx1, and Msx1 in vitro. These findings indicate that Mage-D1 is play an important regulatory role in normal mineralization of teeth. p75NTR, Dlx1, and Msx1 seem to be closely related to the underlying mechanism of Mage-D1 action."

基金机构：National Natural Science Foundation of China [81970906]; First batch of key Disciplines On Public Health in Chongqing

基金资助正文：This study was supported by the National Natural Science Foundation of China (Grant no. 81970906) and the First batch of key Disciplines On Public Health in Chongqing. All the experiments were performed in the Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences.