Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic Pump

作者全名："Che, Bingchen; Sun, Dan; Yan, Ningzhe; Zhao, Wei; Liu, Yonggang; Jing, Guangyin; Zhang, Ce"

作者地址："[Che, Bingchen; Sun, Dan; Zhao, Wei; Zhang, Ce] Northwest Univ, Inst Photon & Photon Technol, State Key Lab Photon Technol Western China Energy, Xian 710069, Peoples R China; [Che, Bingchen; Yan, Ningzhe; Jing, Guangyin] Northwest Univ, Sch Phys, Xian 710069, Peoples R China; [Liu, Yonggang] Chongqing Med Univ, Lab Stem Cell & Tissue Engn, Chongqing, Peoples R China"

通信作者："Zhang, C (通讯作者)，Northwest Univ, Inst Photon & Photon Technol, State Key Lab Photon Technol Western China Energy, Xian 710069, Peoples R China."

来源：ADVANCED INTELLIGENT SYSTEMS

ESI学科分类：　

WOS号：WOS:000934607600001

JCR分区：Q1

影响因子：7.4

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：dynamic flow; peristaltic pumps; ultralow flow rates

摘要："Precise control over flow rate and flow pattern is critical for investigating the contribution of shear force in cellular responses, biofluidic transport, and living matter in a flowing environment. Generation and control over the ultralow flow fields at microscale are, however, difficult, due to thermal agitation and fluctuations in the surrounding environment. Herein, pa novel microfluidic flow field generator is proposed, capable of generating stable flow field down to the limit of thermal noise. Integrated with a customized MATLAB program controlling timely open-close of 18 monolithic PDMS valves, the flow generator outperforms the existing methods by both achievable low flow rate, that is, 0.01 nL s(-1), temporal resolution of flow rate fluctuation down to 100 ms, and provides complex flow patterns. Moreover, unlike external devices (e.g., the syringe pump), the design can be easily integrated into any fluidic chip and sealed from the disturbance of experimental conditions, for example, the airflow and the movement of connected pipelines. The methodology is, therefore, suitable for biological and biophysical studies requiring a well-defined flowing environment."

基金机构：National Natural Science Foundation of China [51927804]; China Scholarship Council [202106970013]; Natural Science Foundation of Shaanxi Province [2021JM-301]

基金资助正文："B.C. and D.S. contributed equally to this work. This work was supported by the National Natural Science Foundation of China (51927804), China Scholarship Council (202106970013), and Natural Science Foundation of Shaanxi Province (2021JM-301). Special thanks to technical support for live-cell imaging from Ronglight Co. Ltd, China."