Mesoporous Nanozyme-Enhanced DNA Tetrahedron Electrochemiluminescent Biosensor with Three-Dimensional Walking Nanomotor-Mediated CRISPR/Cas12a for Ultrasensitive Detection of Exosomal microRNA

作者全名："Shen, Bo; Li, Li; Liu, Changjin; Li, Xinmin; Li, Xinyu; Cheng, Xiaoxue; Wu, Haiping; Yang, Tiantian; Cheng, Wei; Ding, Shijia"

作者地址："[Shen, Bo; Li, Xinyu; Wu, Haiping; Yang, Tiantian; Cheng, Wei; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China; [Shen, Bo; Li, Xinmin] Chongqing Hosp Tradit Chinese Med, Chongqing Key Lab Sichuan Chongqing Coconstruct Di, Chongqing 400021, Peoples R China; [Li, Li] Chongqing Gen Hosp, Dept Lab Med, Chongqing 401147, Peoples R China; [Liu, Changjin] Fifth Peoples Hosp Chongqing, Dept Lab Med, Chongqing 400062, Peoples R China; [Cheng, Xiaoxue] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China"

通信作者："Ding, SJ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China."

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000935984300001

JCR分区：Q1

影响因子：7.4

年份：2023

卷号：95

期号：9

开始页：4486

结束页：4495

文献类型：Article

关键词：　

摘要："Exosomal microRNAs (exomiRNAs) have emerged as ideal biomarkers for early clinical diagnostics. The accurate detection of exomiRNAs plays a crucial role in facilitating clinical applications. Herein, an ultrasensitive electrochemiluminescent (ECL) biosensor was constructed using three-dimensional (3D) walking nanomotor-mediated CRISPR/Cas12a and tetrahedral DNA nanostructures (TDNs)-modified nanoemitters (TCPP-Fe@HMUiO@Au-ABEI) for exomiR-155 detection. Initially, the 3D walking nanomotor-mediated CRISPR/Cas12a strategy could effectively convert the target exomiR-155 into amplified biological signals for improving the sensitivity and specificity. Then, TCPP-Fe@HMUiO@Au nanozymes with excellent catalytic performance were used to amplify ECL signals because of the enhanced mass transfer and increased catalytic active sites, originating from its high surface areas (601.83 m2/g), average pore size (3.46 nm), and large pore volumes (0.52 cm3/g). Meanwhile, the TDNs as the scaffold to fabricate ""bottom-up"" anchor bioprobes could improve the trans-cleavage efficiency of Cas12a. Consequently, this biosensor achieved the limit of detection down to 273.20 aM ranging from 1.0 fM to 1.0 nM. Furthermore, the biosensor could discriminate breast cancer patients evidently by analyzing exomiR-155, and these results conformed to that of qRT-PCR. Thus, this work provides a promising tool for early clinical diagnostics."

基金机构："National Natural Science Foundation of China [82272432, 82002257]; Natural Science Foundation Project of Chongqing [cstc2021jcyj-msxmX0325]; Joint Project of Chongqing Health Commission and Science and Technology Bureau [2022MSXM106]"

基金资助正文："This study was supported by the National Natural Science Foundation of China (82272432 and 82002257) , the Natural Science Foundation Project of Chongqing (cstc2021jcyj-msxmX0325) , and the Joint Project of Chongqing Health Commission and Science and Technology Bureau (2022MSXM106) ."