Protein-directed synthesis of fluorescent sulfur quantum dots for highly robust detection of pyrophosphate

作者全名："Gong, Xue; Cai, Qingqing; Zhang, Jiajia; Min, Yuanhong; Fu, Wensheng; Wang, Yi; Zhang, Pu"

作者地址："[Gong, Xue; Cai, Qingqing; Zhang, Jiajia; Min, Yuanhong; Fu, Wensheng; Wang, Yi] Chongqing Normal Univ, Coll Chem, Engn Res Ctr Biotechnol Act Subst, Chongqing Key Lab Green Synth & Applicat,Minist Ed, Chongqing 401331, Peoples R China; [Zhang, Pu] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China"

通信作者："Wang, Y (通讯作者)，Chongqing Normal Univ, Coll Chem, Engn Res Ctr Biotechnol Act Subst, Chongqing Key Lab Green Synth & Applicat,Minist Ed, Chongqing 401331, Peoples R China.; Zhang, P (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：MICROCHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000939369300001

JCR分区：Q1

影响因子：5.7

年份：2023

卷号：190

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：Photoluminescence; Pyrophosphate; Aggregation-induced quenching; Sulfur quantum dots

摘要："Inorganic pyrophosphate anions (PPi) play a key role in various biological processes and act as an essential indicator for physiological function evaluation and disease diagnosis. However, there is still a lack of available approaches for straightforward, robust, and convenient PPi detection. Herein, we design an on-off-on fluorescent switching nanoprobe employing Fe3+-mediated fluorescent sulfur quantum dots (SQDs) for highly robust detection of PPi. The bovine serum protein (BSA)-capped SQDs with fine water dispersibility and good optical stability are synthesized by an H2O2-assisted chemical etching reaction. Specifically, Fe3+ can strongly induce the aggregation of the SQDs into relatively larger sizes, resulting in aggregation-induced fluorescence quenching behavior. PPi can selectively bind with Fe3+ via emulative coordination and in preventing the aggregation of SQDs this is accompanied by recovery of fluorescence. The physicochemical properties of aggregated and disaggregated SQDs have been systematically investigated. Aggregation and disaggregation of the SQDs and the corresponding quenching and recovery of fluorescence occurs and guarantees the high-contrast sensing performance of the SQD system in complex and challenging aquatic environments. Our designed on-off-on nanoswitch holds great potential for the design of elemental quantum dot-based biosensors for the highly robust detection of analytes in the near future."

基金机构："National Natural Science Foundation of China [21775014, 22204012]; Chongqing Innovation Research Group Project [CXQT21015]; Natural Science Foundation of Chongqing [CSTB2022NSCQ-MSX0466]; Startup Founds of Chongqing Normal University [22XLB010]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (no. 21775014 and 22204012), Chongqing Innovation Research Group Project (no. CXQT21015), Natural Science Foundation of Chongqing (no. CSTB2022NSCQ-MSX0466), and Startup Founds of Chongqing Normal University (no. 22XLB010)."