Neuroglobin plays as tumor suppressor by disrupting the stability of GPR35 in colorectal cancer

作者全名："Xiang, Qin; Zhou, Dishu; Xiang, Xinni; Le, Xin; Deng, Chaoqun; Sun, Ran; Li, Chunhong; Pang, Huayang; He, Jin; Zheng, Zeze; Tang, Jun; Peng, Weiyan; Peng, Xi; He, Xiaoqian; Wu, Fan; Qiu, Jingfu; Xu, Yongzhu; Xiang, Tingxiu"

作者地址："[Xiang, Qin; Le, Xin; Deng, Chaoqun; Sun, Ran; Li, Chunhong; He, Jin; Tang, Jun; Peng, Weiyan; Peng, Xi; He, Xiaoqian; Wu, Fan; Xiang, Tingxiu] Chongqing Med Univ, Dept Oncol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Xiang, Qin; Zhou, Dishu; Pang, Huayang; Zheng, Zeze; Xiang, Tingxiu] Chongqing Univ Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Chongqing 400030, Peoples R China; [Qiu, Jingfu] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China; [Xu, Yongzhu] Chongqing Blood Ctr, Chongqing 400015, Peoples R China; [Xiang, Xinni] Sichuan Univ, West China Sch Med, Chengdu 610065, Sichuan, Peoples R China"

通信作者："Xiang, TX (通讯作者)，Chongqing Med Univ, Dept Oncol, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Xiang, TX (通讯作者)，Chongqing Univ Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Chongqing 400030, Peoples R China.; Qiu, JF (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China.; Xu, YZ (通讯作者)，Chongqing Blood Ctr, Chongqing 400015, Peoples R China."

来源：CLINICAL EPIGENETICS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000961427700001

JCR分区：Q1

影响因子：5.7

年份：2023

卷号：15

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：NGB; GPR35; Biomarker; Methylation; Tumor angiogenesis

摘要："BackgroundThe incidence of colorectal cancer (CRC) has increased in recent years. Identification of accurate tumor markers has become the focus of CRC research. Early and frequent DNA methylation tends to occur in cancer. Thus, identifying accurate methylation biomarkers would improve the efficacy of CRC treatment. Neuroglobin (NGB) is involved in neurological and oncological diseases. However, there are currently no reports on epigenetic regulation involvement of NGB in CRC.ResultsNGB was downregulated or silenced in majority CRC tissues and cell lines. The hypermethylation of NGB was detected in tumor tissue, but no or a very low methylation frequency in normal tissues. Overexpression of NGB induced G2/M phase arrest and apoptosis, suppressed proliferation, migration, invasion in vitro, and inhibited CRC tumor growth and angiogenesis in vivo. Isobaric tag for relative and absolute quantitation (Itraq)-based proteomics identified approximately 40% proteins related to cell-cell adhesion, invasion, and tumor vessel formation in the tumor microenvironment, among which GPR35 was proved critical for NGB-regulated tumor angiogenesis suppression in CRC.ConclusionsNGB, an epigenetically silenced factor, inhibits metastasis through the GPR35 in CRC. It is expected to grow into a potential cancer risk assessment factor and a valuable biomarker for early diagnosis and prognosis assessment of CRC."

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