Target response controlled enzyme activity switch for multimodal biosensing detection
作者全名："Zhang, Lu; Wu, Haiping; Chen, Yirong; Zhang, Songzhi; Song, Mingxuan; Liu, Changjin; Li, Jia; Cheng, Wei; Ding, Shijia"
作者地址："[Zhang, Lu; Wu, Haiping; Chen, Yirong; Zhang, Songzhi; Song, Mingxuan; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Liu, Changjin] Fifth Peoples Hosp Chongqing, Dept Lab Med, Chongqing 400062, Peoples R China; [Li, Jia; Cheng, Wei] Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China"
通信作者："Ding, SJ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China.; Li, J; Cheng, W (通讯作者)，Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China."
来源：JOURNAL OF NANOBIOTECHNOLOGY
ESI学科分类：BIOLOGY & BIOCHEMISTRY
关键词：Hemin; Controlled enzyme activity; Dimerization inactivation; G4-Hemin DNAzyme; Multimodal detection
摘要："How to achieve delicate regulation of enzyme activity and empower it with more roles is the peak in the field of enzyme catalysis research. Traditional proteases or novel nano-enzymes are unable to achieve stimulus-responsive activity modulation due to their own structural limitations. Here, we propose a novel (C) under bar ontrollable (E) under bar nzyme (A) under bar ctivity (S) under bar witch, CEAS, based on hemin aggregation regulation, to deeply explore its regulatory mechanism and develop multimodal biosensing applications. The core of CEAS relies on the dimerizable inactivation of catalytically active center hemin and utilizes a DNA template to orderly guide the G4-Hemin DNAzyme to tightly bind to DNA-Hemin, thereby shutting down the catalytic ability. By customizing the design of the guide template, different target stimulus responses lead to hemin dimerization dissociation and restore the synergistic catalysis of G4-Hemin and DNA-Hemin, thus achieving a target-regulated enzymatic activity switch. Moreover, the programmability of CEAS allowed it easy to couple with a variety of DNA recognition and amplification techniques, thus developing a series of visual protein detection systems and highly sensitive fluorescent detection systems with excellent bioanalytical performance. Therefore, the construction of CEAS is expected to break the limitation of conventional enzymes that cannot be targetable regulated, thus enabling customizable enzymatic reaction systems and providing a new paradigm for controllable enzyme activities."
基金机构：National Natural Science Foundation of China ; Chongqing Talent-Innovation Leader Project [cstc2022y-cjh-bgzxm0001]; Key Laboratory of Tropical Disease Prevention and Control of the National Health Commission of Hainan Medical College [2022NHCTDCKFKT21002]; Chongqing Medical University Graduate Talent Training Program [BJRC202104]
基金资助正文："This work was funded by the National Natural Science Foundation of China (81873980), the Chongqing Talent-Innovation Leader Project (cstc2022y-cjh-bgzxm0001), Open project of the Key Laboratory of Tropical Disease Prevention and Control of the National Health Commission of Hainan Medical College (2022NHCTDCKFKT21002), Chongqing Medical University Graduate Talent Training Program (BJRC202104)"