Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study

作者全名："Chen, Xia; Zhang, Panyang; Zhang, Yajuan; Fan, Shijun; Wei, Yan; Yang, Zhifan; Wang, Fengchao; Peng, Xi"

作者地址："[Chen, Xia; Zhang, Panyang; Zhang, Yajuan; Fan, Shijun; Wei, Yan; Peng, Xi] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Clin Med Res Ctr, Chongqing 400038, Peoples R China; [Yang, Zhifan; Wang, Fengchao] Third Mil Med Univ, Inst Combined Injury, State Key Lab Trauma Burns & Combined Injury, Coll Prevent Med,Army Med Univ, Chongqing 400038, Peoples R China; [Peng, Xi] Third Mil Med Univ, Army Med Univ, State Key Lab Trauma Burns & Combined Injury, Chongqing 400038, Peoples R China"

通信作者："Peng, X (通讯作者)，Third Mil Med Univ, Army Med Univ, Southwest Hosp, Clin Med Res Ctr, Chongqing 400038, Peoples R China.; Wang, FC (通讯作者)，Third Mil Med Univ, Inst Combined Injury, State Key Lab Trauma Burns & Combined Injury, Coll Prevent Med,Army Med Univ, Chongqing 400038, Peoples R China.; Peng, X (通讯作者)，Third Mil Med Univ, Army Med Univ, State Key Lab Trauma Burns & Combined Injury, Chongqing 400038, Peoples R China."

来源：NUTRIENTS

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:000970345200001

JCR分区：Q1

影响因子：5.9

年份：2023

卷号：15

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：glutamine; intestinal stem cells; crypt; proliferation; burns

摘要："Burn injury is a common form of traumatic injury that leads to high mortality worldwide. A severe burn injury usually induces gut barrier dysfunction, partially resulting from the impairment in the proliferation and self-renewal of intestinal stem cells (ISCs) post burns. As a main energy substance of small intestinal enterocytes, glutamine (Gln) is important for intestinal cell viability and growth, while its roles in ISCs-induced regeneration after burns are still unclear. To demonstrate the potential effects of Gln in improving ISCs proliferation and alleviating burn-induced intestinal injury, in this study, we verified that Gln significantly alleviated small intestine injury in burned mice model. It showed that Gln could significantly decrease the ferroptosis of crypt cells in the ileum, promote the proliferation of ISCs, and repair the crypt. These effects of Gln were also confirmed in the mouse small intestine organoids model. Further research found that Yes-associated protein (YAP) is suppressed after burn injury, and Gln could improve cell proliferation and accelerate the renewal of the damaged intestinal mucosal barrier after burns by activating YAP. YAP is closely associated with the changes in intestinal stem cell proliferation after burn injury and could be served as a potential target for severe burns."

基金机构："National Natural Science Foundation of China [82172202]; Innovative Leading Talents Project of Chongqing, China [cstc2022ycjh-bgzxm0148, cstc2018jcyj-yszxX0004]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (No. 82172202), and the Innovative Leading Talents Project of Chongqing, China (NO. cstc2022ycjh-bgzxm0148 and cstc2018jcyj-yszxX0004)."