Toehold-me diate d strand displacement reaction-propelled cascade DNAzyme amplifier for microRNA let-7a detection
作者全名："Wang, Na; Jiang, Yongjian; Nie, Kunhan; Li, Di; Liu, Hui; Wang, Jian; Huang, Chengzhi; Li, Chunmei"
作者地址："[Wang, Na; Jiang, Yongjian; Nie, Kunhan; Liu, Hui; Wang, Jian; Huang, Chengzhi; Li, Chunmei] Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China; [Li, Di] Chongqing Med Univ, Affiliated Hosp 2, Chongqing 400010, Peoples R China"
通信作者："Li, CM (通讯作者)，Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China."
来源：CHINESE CHEMICAL LETTERS
关键词：DNAzyme amplifier; Toehold-mediated strand displacement reaction; Signal amplification; let-7a; Cancer marker
摘要："DNAzyme amplifiers have been extensively explored as a useful sensing platform, but single DNAzyme amplifier is limited in biosensing applications by its low sensitivity. Herein, a cascade DNAzyme amplifier was designed by exploiting concurrent amplification cycle principles of toehold-mediated strand displacement reaction (TSDR) and Zn2+-assisted DNAzyme cycle with lower cost and simpler procedures. Compared with single DNAzyme amplifier, the proposed TSDR-propelled cascade DNAzyme amplifier exhibited higher sensitivity by releasing more DNAzyme through TSDR to cleave substrate strand during the DNAzyme cycle. Base on this, let-7a could be sensitively detected in the range of 5-50 nmol/L with a detection limit of 64 pmol/L. Furthermore, the dual signal amplification strategy of the cascade DNAzyme amplifier exhibited excellent selectivity to distinguish single-base mismatched DNA strands, which has been successfully applied to the determination of let-7a in blood serum, showing high promise in early cancer diagnosis. (c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences."
基金机构："National Natural Science Foundation of China (NSFC) [22074124, 22134005]; fund of Fundamental Research Funds for the Cen-tral Universities [XDJK2020TY001]; Chongqing Talents Program for Outstanding Scientists [cstc2021ycjh-bgzxm0178]; Chongqing Graduate Student Scientific Research Innovation Project [CYB21119]"
基金资助正文："This work was financially supported by the National Natural Science Foundation of China (NSFC, Nos. 22074124 and 22134005) , the fund of Fundamental Research Funds for the Cen-tral Universities (No. XDJK2020TY001) , Chongqing Talents Program for Outstanding Scientists (No. cstc2021ycjh-bgzxm0178) and the Chongqing Graduate Student Scientific Research Innovation Project (No. CYB21119) ."