Downregulation of ROR2 attenuates LPS-induced A549 cell injury through JNK and ERK signaling pathways

作者全名："Wang, Zhonglin; Yang, Liu"

作者地址："[Wang, Zhonglin; Yang, Liu] Chongqing Med Univ, Dept Anesthesiol, Yongchuan Hosp, Yongchuan, Peoples R China; [Yang, Liu] Chongqing Med Univ, Dept Anesthesiol, Yongchuan Hosp, 439 Xuanhua Rd, Yongchuan 402160, Peoples R China"

通信作者："Yang, L (通讯作者)，Chongqing Med Univ, Dept Anesthesiol, Yongchuan Hosp, 439 Xuanhua Rd, Yongchuan 402160, Peoples R China."

来源：IMMUNITY INFLAMMATION AND DISEASE

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000975080600001

JCR分区：Q4

影响因子：3.2

年份：2023

卷号：11

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：acute lung injury; ERK; JNK; ROR2; small interfering RNA

摘要："BackgroundWe aimed to determine whether receptor tyrosine kinase-like orphan receptor 2 (ROR2) is involved in the occurrence of acute lung injury (ALI) by an animal study and explore the effect of ROR2 downregulation on lipopolysaccharide (LPS)-treated human lung carcinoma A549 cells by a cytological study. MethodsMurine models of ALI were successfully constructed by intratracheal instillation of LPS. Meanwhile, A549 cell line stimulated with LPS was used for a cytological study. The expression of ROR2 and its effect on proliferation, cell cycle, apoptosis, and inflammation were detected. ResultsIt was found that LPS administration markedly inhibited the cell proliferation, resulted in cell cycle arrest at G1 phage, elevated levels of pro-inflammatory cytokines and apoptosis rate of A549 cells. However, LPS-mediated adverse effects mentioned above were significantly ameliorated by downregulation of ROR2 in comparison with LPS treatment. In addition, administration of ROR2 siRNA notably decreased the phosphorylation level of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in LPS-challenged A549 cells. ConclusionsThus, the present data indicate that downregulation of ROR2 may decrease LPS-induced inflammatory responses and cell apoptosis through inhibiting JNK and ERK signaling pathway, which attenuates ALI."

基金机构：Yongchuan Hospital Affiliated to Chongqing Medical University Foundation [YJZQN201520]

基金资助正文：ACKNOWLEDGMENTS The current study was supported by Yongchuan Hospital Affiliated to Chongqing Medical University Foundation (grant no. YJZQN201520).