Alleviation of DSS-induced colitis in mice by a new-isolated Lactobacillus acidophilus C4

作者全名："Liu, Qianqian; Jian, Wenwen; Wang, Lu; Yang, Shenglin; Niu, Yutian; Xie, ShuaiJing; Hayer, Kim; Chen, Kun; Zhang, Yi; Guo, Yanan; Tu, Zeng"

作者地址："[Liu, Qianqian; Jian, Wenwen; Wang, Lu; Yang, Shenglin; Xie, ShuaiJing; Tu, Zeng] Chongqing Med Univ, Coll Basic Med Sci, Dept Pathogen Biol, Chongqing, Peoples R China; [Niu, Yutian; Zhang, Yi; Guo, Yanan] Chongqing Med Univ, Int Med Coll, Chongqing, Peoples R China; [Hayer, Kim] Univ Leicester, Leicester Med Sch, Leicester, England; [Chen, Kun] Chongqing Med Univ, Coll Foreign Languages, Chongqing, Peoples R China"

通信作者："Tu, Z (通讯作者)，Chongqing Med Univ, Coll Basic Med Sci, Dept Pathogen Biol, Chongqing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000979889100001

JCR分区：Q2

影响因子：5.2

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Lactobacillus acidophilus; ulcerative colitis; probiotics; intestinal barriers; intestinal microbiota

摘要："IntroductionProbiotic is adjuvant therapy for traditional drug treatment of ulcerative colitis (UC). In the present study, Lactobacillus acidophilus C4 with high acid and bile salt resistance has been isolated and screened, and the beneficial effect of L. acidophilus C4 on Dextran Sulfate Sodium (DSS)-induced colitis in mice has been evaluated. Our data showed that oral administration of L. acidophilus C4 remarkably alleviated colitis symptoms in mice and minimized colon tissue damage. MethodsTo elucidate the underlying mechanism, we have investigated the levels of inflammatory cytokines and intestinal tight junction (TJ) related proteins (occludin and ZO-1) in colon tissue, as well as the intestinal microbiota and short-chain fatty acids (SCFAs) in feces. ResultsCompared to the DSS group, the inflammatory cytokines IL-1 beta, IL-6, and TNF-alpha in L. acidophilus C4 group were reduced, while the antioxidant enzymes superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) were found to be elevated. In addition, proteins linked to TJ were elevated after L. acidophilus C4 intervention. Further study revealed that L. acidophilus C4 reversed the decrease in intestinal microbiota diversity caused by colitis and promoted the levels of SCFAs. DiscussionThis study demonstrate that L. acidophilus C4 effectively alleviated DSS-induced colitis in mice by repairing the mucosal barrier and maintaining the intestinal microecological balance. L. acidophilus C4 could be of great potential for colitis therapy."

基金机构：　

基金资助正文：