Fast three-dimensional DNA walker based on space double-confinement catalytic hairpin assembly for ultrasensitive homogeneous electrochemiluminescence detection of sonic hedgehog
作者全名："Ai, Zhujun; Li, Xinmin; Chen, Rui; Han, Daobin; Bai, Huijie; Yan, Shaoying; Gu, Pengcheng; Wu, Haiping; Ding, Shijia; Chen, Junman; Tang, Hua"
作者地址："[Ai, Zhujun; Li, Xinmin; Chen, Rui; Bai, Huijie; Wu, Haiping; Ding, Shijia; Chen, Junman] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Tang, Hua] Chongqing Med Univ, Affiliated Hosp 2, Dept Infect Dis, Key Lab Mol Biol Infect Dis,Minist Educ,Inst Viral, Chongqing 400016, Peoples R China; [Han, Daobin] Shandong Univ, Hosp 2, Med Lab Ctr, Jinan 250000, Shandong, Peoples R China; [Yan, Shaoying] Nanchang Univ, Affiliated Hosp 1, Dept Clin Lab, Nanchang 330006, Jiangxi, Peoples R China; [Gu, Pengcheng] Chongqing Med Univ, Lab Pharm & Chem, Lab Teaching & Management Ctr, Chongqing 400016, Peoples R China"
通信作者："Chen, JM (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China.; Tang, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Infect Dis, Key Lab Mol Biol Infect Dis,Minist Educ,Inst Viral, Chongqing 400016, Peoples R China."
来源：SENSORS AND ACTUATORS B-CHEMICAL
关键词：DNA walker; Space double confinement; Catalytic hairpin assembly; Homogeneous electrochemiluminescence; biosensor; Sonic hedgehog
摘要："DNA walkers have been widely used to build biosensing systems. However, their moving efficiency was limited, which has hindered practical applications. Here, we for the first time integrated localized CHA with nanoscale AuNPs scaffolds to achieve space double-confined catalytic hairpin assembly (SDCHA) for developing a fast three-dimensional DNA walker. Driven by spatially proximal displacement, the walker possessed a high walking speed, which was about 8 times, 9 times and 23 times faster than LCHA, CHA-AuNPs and conventional CHA, respectively. Moreover, the walking time was about 1.4-8.3 times shorter than most recently reported DNA walkers and space-confined strand displacement reactions. By further integrating it with magnetic probes and g-C3N4 nanosheets, an ultrasensitive and enzyme-free homogeneous electrochemiluminescence (Ho-ECL) apta-sensor was firstly constructed for sonic hedgehog (SHh) detection. Profiting from this tailored integration, the Ho-ECL aptasensor avoided multiple interferences from complex milieu as well as tedious procedures of electrode modifying and washing, showing good signal stability, repeatability and reliability. It was also proved to achieve ultrasensitive SHh detection with the detection limit as low as 13.5 fM, superior to previous research on SHh detection. This work might broaden ideas for the construction of fast DNA walkers and facile Ho-ECL bio-analytical methods for clinical diagnosis."
基金机构："Key Laboratory of Infectious Diseases, CQMU ; Youth Fund of Natural Science Foundation of Shandong Province [ZR2021QH055]; Province Natural Science Foundation of Jiangxi Province [20202BABL216040]; Science and Technology Plan Project of the Education Department of Jiangxi Province [GJJ190008]; Natural Science Foundation of Chongqing (Postdoctoral Foundation) [cstc2021jcyj-bsh X0153]; Chongqing Talent-Innovation Leader Project [cstc2022ycjh-bgzxm0001]"
基金资助正文："This work was supported by the Key Laboratory of Infectious Diseases, CQMU (202004); Youth Fund of Natural Science Foundation of Shandong Province (ZR2021QH055); the Province Natural Science Foundation of Jiangxi Province (20202BABL216040); the Science and Technology Plan Project of the Education Department of Jiangxi Province (GJJ190008); the Natural Science Foundation of Chongqing (Postdoctoral Foundation, cstc2021jcyj-bsh X0153); and the Chongqing Talent-Innovation Leader Project (No:cstc2022ycjh-bgzxm0001) ."