Effects of chronic sleep restriction on the neuro-phenotypes of Ctnnd2 knockout mice

作者全名："Xu, Man; Wang, Xiaoya; Wang, Luyi; Wang, Shali; Deng, Jing; Wang, Yan; Li, Yingbo; Pan, Sen; Liao, Ailing; Tao, Yihao; Tan, Shujiang"

作者地址："[Xu, Man; Tan, Shujiang] Chongqing Univ, Dept Pediat, Fuling Hosp, Chongqing, Peoples R China; [Xu, Man; Wang, Xiaoya; Wang, Luyi; Wang, Shali; Deng, Jing; Wang, Yan; Li, Yingbo; Pan, Sen; Liao, Ailing] Chongqing Med Univ, Inst Neurosci, Sch Basic Med Sci, Dept Physiol, Chongqing, Peoples R China; [Wang, Xiaoya] North Sichuan Med Coll, Dept Pathol, Affiliated Hosp, Nanchong, Sichuan, Peoples R China; [Wang, Luyi] Chongqing Univ, Dept Nucl Med, Fuling Hosp, Chongqing, Peoples R China; [Deng, Jing] Army Med Univ, Xinqiao Hosp, Dept Neurosurg, Chongqing, Peoples R China; [Pan, Sen] Chongqing Univ, Dept Urol, Fuling Hosp, Chongqing, Peoples R China; [Liao, Ailing] Chongqing Populat & Family Planning Sci & Technol, NHC Key Lab Birth Defects & Reprod Hlth, Chongqing, Peoples R China; [Tao, Yihao] Chongqing Med Univ, Affiliated Hosp 2, Dept Neurosurg, Chongqing, Peoples R China"

通信作者："Tan, SJ (通讯作者)，Chongqing Univ, Dept Pediat, Fuling Hosp, Chongqing, Peoples R China.; Tao, YH (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Neurosurg, Chongqing, Peoples R China."

来源：BRAIN AND BEHAVIOR

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000992673300001

JCR分区：Q2

影响因子：3.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：synapse; sleep restriction; dendritic spines; Ctnnd2; cognition; autism

摘要："IntroductionSleep abnormalities are highly correlated with neurodevelopmental disorders, such as intellectual disability, attention deficit hyperactivity disorder, and autism spectrum disorders (ASD). The severity of behavioral abnormalities is correlated with the presence of sleep abnormalities. Based on previous research, we investigated that Ctnnd2 gene deletion in mice lead to ASD-like behaviors and cognitive defects. Given the importance of sleep in individuals with ASD, this study aimed to determine the effects of chronic sleep restriction (SR) on wild-type (WT) mice and on Ctnnd2 deletion-induced, neurologically related phenotypes in mice. MethodWT and Ctnnd2 knockout (KO) mice were both subjected to manual SR (5 h per day) for 21 consecutively days separately, then we compared neurologically related phenotypes of WT mice, WT mice subjected to SR, KO mice, and KO mice subjected to SR using a three-chamber assay, direct social interaction test, open-field test, Morris water maze, Golgi staining, and Western blotting. ResultsThe effects of SR on WT and KO mice were different. After SR, social ability and cognition were impaired in both WT and KO mice. Repetitive behaviors were increased, and exploration abilities were decreased in KO mice but not in WT mice. Moreover, SR reduced the density and area of mushroom-type dendritic spines in WT rather than KO mice. Finally, the PI3K/Akt-mTOR pathway was found to be involved in the effects induced by SR-impaired phenotypes in WT and KO mice. ConclusionOverall, results of the present study may have implications for the role of disrupted sleep in patients with CTNND2 gene-related autism and the evolution of neurodevelopmental disorders."

基金机构：Chongqing Regional Key Discipline of Medicine [zdxk201718]; Chongqing Natural Science Foundation [cstc2020jcyj-msxmX0225]

基金资助正文：ACKNOWLEDGMENTS This work was supported by the Chongqing Regional Key Discipline of Medicine (Grant No. zdxk201718) and Chongqing Natural Science Foundation (Grant No. cstc2020jcyj-msxmX0225).