Ginsenoside Rg1 ameliorates Alzheimer's disease pathology via restoring mitophagy

作者全名："Wang, Ni; Yang, Junyan; Chen, Ruijun; Liu, Yunyun; Liu, Shunjie; Pan, Yining; Lei, Qingfeng; Wang, Yuzhou; He, Lu; Song, Youqiang; Li, Zhong"

作者地址："[Wang, Ni; Liu, Yunyun; Liu, Shunjie; Lei, Qingfeng; Wang, Yuzhou; He, Lu; Song, Youqiang; Li, Zhong] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Neurol, Guangzhou, Peoples R China; [Wang, Ni; Chen, Ruijun; Pan, Yining; Song, Youqiang] Univ Hong Kong, Li Ka Shing Fac Med, Sch Biomed Sci, Hong Kong, Peoples R China; [Yang, Junyan] Chongqing Med Univ, Stomatol Hosp, Dept Clin Lab, Chongqing, Peoples R China; [Li, Zhong] Sun Yat Sen Univ, Zhongshan Sch Med, Guangdong Prov Key Lab Brain Funct & Dis, Guangzhou, Peoples R China; [Li, Zhong] Sun Yat Sen Univ, Shenzhen Res Inst, Southern Dist Nanshan Sci Pk, Shenzhen, Peoples R China"

通信作者："Song, YQ; Li, Z (通讯作者)，Sun Yat Sen Univ, Affiliated Hosp 6, Dept Neurol, Guangzhou, Peoples R China."

来源：JOURNAL OF GINSENG RESEARCH

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001001820600001

JCR分区：Q1

影响因子：6.3

年份：2023

卷号：47

期号：3

开始页：448

结束页：457

文献类型：Article

关键词：Alzheimer's disease; ginseng; ginsenoside Rg1; mitophagy; Parkin

摘要："Background: Alzheimer's disease (AD) is a common form of dementia, and impaired mitophagy is a hallmark of AD. Mitophagy is mitochondrial-specific autophagy. Ginsenosides from Ginseng involve in autophagy in cancer. Ginsenoside Rg1 (Rg1 hereafter), a single compound of Ginseng, has neuroprotective effects on AD. However, few studies have reported whether Rg1 can ameliorate AD pathology by regulating mitophagy. Methods: Human SH-SY5Y cell and a 5XFAD mouse model were used to investigate the effects of Rg1. Rg1 (1 mu M) was added to 13-amyloid oligomer (A13O)-induced or APPswe-overexpressed cell models for 24 hours. 5XFAD mouse models were intraperitoneally injected with Rg1 (10 mg/kg/d) for 30 days. Expression levels of mitophagy-related markers were analyzed by western blot and immunofluorescent staining. Cognitive function was assessed by Morris water maze. Mitophagic events were observed using transmission electron microscopy, western blot, and immunofluorescent staining from mouse hippocampus. The activation of the PINK1/Parkin pathway was examined using an immunoprecipitation assay. Results: Rg1 could restore mitophagy and ameliorate memory deficits in the AD cellular and/or mouse model through the PINK1-Parkin pathway. Moreover, Rg1 might induce microglial phagocytosis to reduce 13-amyloid (A13) deposits in the hippocampus of AD mice. Conclusion: Our studies demonstrate the neuroprotective mechanism of ginsenoside Rg1 in AD models. Rg1 induces PINK-Parkin mediated mitophagy and ameliorates memory deficits in 5XFAD mouse models. (c) 2022 The Korean Society of Ginseng. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)."

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