A convenient and economical strategy for multiple-target electrochemiluminescence detection using peroxydisulfate solution

作者全名："Liu, Mei-Ling; He, Xiao-Jing; Li, Yi; Zhao, Mei-Ling; Zhuo, Ying"

作者地址："[Liu, Mei-Ling; He, Xiao-Jing; Li, Yi] Chongqing Med Univ, Affiliated Hosp 2, Dept Radiol, Chongqing, Peoples R China; [Zhao, Mei-Ling; Zhuo, Ying] Southwest Univ, Coll Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing, Peoples R China"

通信作者："He, XJ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Radiol, Chongqing, Peoples R China.; Zhuo, Y (通讯作者)，Southwest Univ, Coll Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing, Peoples R China."

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001011125400003

JCR分区：Q1

影响因子：5.6

年份：2023

卷号：251

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Electrochemiluminescence; Multiple-target detection; Bi-enzyme cascade catalysis; Peroxydisulfate; Aptasensor

摘要："As various aptasensors are adopted in clinical diagnosis, the development of convenient multiple-target deteromination is a field of ever-increasing interests. Herein, a label-free and amplified electrochemiluminescence (ECL) sensing platform was constructed to detect multiple targets of hemin, glucose and thrombin (TB) using peroxydisulfate (S2O82-) solution, which was one of the most convenient and economical ECL systems. It was worth mentioning that the target-induced bi-enzyme cascade catalysis reaction was developed to increase the ECL response strongly of S2O82- solution due to the production of (O-1(2))(2)* from the inter-reaction between reactive oxygen species (ROS) and sulfate radical (SO4 center dot-). Specifically, with the layer-by-layer assembly of multi-walled carbon nanotubes (MWCNTs), glucose oxidase (GOx) and gold nanoparticles (AuNPs) as the interface, the guanine-rich (G-rich) thrombin aptamer (TBA) was anchored for hemin (target 1) detection, due to the electrocatalysis of hemin/G-quadruplex as a horseradish peroxidase mimicking DNAzyme (HRP-DNAzyme) towards dissolved oxygen for ROS generation. Second, in the presence of glucose (target 2), the ECL intensity was improved because glucose was the substrate of the bi-enzyme cascade catalysis reaction. Third, when TB (target 3) was sequentially incubated based on the above-mentioned aptasensor, the bi-enzyme catalysis was inhibited to decrease the ECL signal, due to the steric hindrance effect of the TB protein. As a result, the aptasensor achieved the nanomolar detection for hemin (3.33 nM), the micromolar detection for glucose (0.33 mu M) and the femtomolar detection for TB (3.33 fM), respectively."

基金机构：General program of Chongqing Natural Science Foundation [cstc2019jcyj-msxmX0073]; Science and health joint medical research project of Chongqing [2019GDRC011]; High-level Medical Reserved Personnel Training Project of Chongqing and Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University

基金资助正文："This work was supported by the General program of Chongqing Natural Science Foundation (cstc2019jcyj-msxmX0073), Science and health joint medical research project of Chongqing (2019GDRC011), High-level Medical Reserved Personnel Training Project of Chongqing and Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University."