Effects of electric charge on fracture healing

作者全名："He, Ling; Yao, Yingling; Wang, Nan; Nan, Guoxin"

作者地址："[He, Ling; Yao, Yingling; Wang, Nan; Nan, Guoxin] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, Dept Orthopaed,Childrens Hosp,Minist Educ, Chongqing 400014, Peoples R China; [He, Ling; Yao, Yingling; Wang, Nan; Nan, Guoxin] Chongqing Engn Res Ctr Stem Cell Therapy, Chongqing, Peoples R China; [He, Ling; Yao, Yingling; Wang, Nan; Nan, Guoxin] Chongqing Med Univ, Dept Orthopaed, Childrens Hosp, Chongqing, Peoples R China"

通信作者："Nan, GX (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, Dept Orthopaed,Childrens Hosp,Minist Educ, Chongqing 400014, Peoples R China.; Nan, GX (通讯作者)，Chongqing Engn Res Ctr Stem Cell Therapy, Chongqing, Peoples R China.; Nan, GX (通讯作者)，Chongqing Med Univ, Dept Orthopaed, Childrens Hosp, Chongqing, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001012301900019

JCR分区：Q1

影响因子：4.6

年份：2022

卷号：12

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Fracture nonunion is a common and challenging complication. Although direct current stimulation has been suggested to promote fracture healing, differences in cell density near the positive and negative electrodes have been reported during direct current stimulation. This study aimed to explore the effects of these differences on osteoblast proliferation and fracture healing. MC3T3-E1 cells were stimulated by positive and negative charges to observe cell proliferation, apoptosis, and osteogenic factor expression in vitro, while positive and negative charges were connected to the Kirschner wires of the fractures in an in vivo double-toe fracture model in New Zealand white rabbits and fracture healing was assessed in digital radiography (DR) examinations performed on days 1, 15, 30. Bone tissue samples of all rabbits were analysed histologically after the last examination. The results showed that in comparison with the control group, after DC stimulation, the number of cells near the positive electrode decreased significantly (P < 0.05), apoptosis increased (P < 0.05), the expression of osteocalcin, osteoblast-specific genes, and osteonectin decreased significantly near the positive electrode (P < 0.05) and increased significantly at the negative electrode (P < 0.05). The fracture at the positive electrode junction of New Zealand white rabbits did not heal. Histomorphological analysis showed more bone trabeculae and calcified bone in the bone tissue sections of the control group and the negative electrode group than in the positive electrode group. The bone trabeculae were thick and showed good connections. However, positive charge inhibited osteoblast proliferation and a positive charge at fracture sites did not favour fracture healing. Thus, a positive charge near the fracture site may be a reason for fracture nonunion."

基金机构：　

基金资助正文：