Temperature-Promoted Giant Unilamellar Vesicle (GUV) Aggregation: A Way of Multicellular Formation

作者全名："Wang, Xinmao; Zhang, Yangruizi; Xie, Maobin; Wang, Zhibiao; Qiao, Hai"

作者地址："[Wang, Xinmao; Zhang, Yangruizi; Xie, Maobin; Wang, Zhibiao; Qiao, Hai] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Wang, Xinmao; Zhang, Yangruizi; Xie, Maobin; Wang, Zhibiao; Qiao, Hai] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China"

通信作者："Qiao, H (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China.; Qiao, H (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China."

来源：CURRENT ISSUES IN MOLECULAR BIOLOGY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001020129400001

JCR分区：Q4

影响因子：3.1

年份：2023

卷号：45

期号：5

开始页：3757

结束页：3771

文献类型：Article

关键词：giant unilamellar vesicles; aggregation; phospholipid; zeta potential; headgroup reorientation

摘要："The evolution of unicellular to multicellular life is considered to be an important step in the origin of life, and it is crucial to study the influence of environmental factors on this process through cell models in the laboratory. In this paper, we used giant unilamellar vesicles (GUVs) as a cell model to investigate the relationship between environmental temperature changes and the evolution of unicellular to multicellular life. The zeta potential of GUVs and the conformation of the headgroup of phospholipid molecules at different temperatures were examined using phase analysis light scattering (PALS) and attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), respectively. In addition, the effect of increasing temperature on the aggregation of GUVs was further investigated in ionic solutions, and the possible mechanisms involved were explored. The results showed that increasing temperature reduced the repulsive forces between cells models and promoted their aggregation. This study could effectively contribute to our understanding of the evolution of primitive unicellular to multicellular life."

基金机构：General Project of Chongqing Natural Science Foundation [cstc2021jcyj-msxmX0550]

基金资助正文：This research was funded by General Project of Chongqing Natural Science Foundation (Grant No. cstc2021jcyj-msxmX0550).