Enzyme-free dual amplification biosensor based on functional nucleic acid and CDs/CoOOH for detection of leukemia fusion gene

作者全名："Shen, Chenlan; Zhong, Liang; Wan, Peng; Jia, Hengke; Liu, Beizhong"

作者地址："[Shen, Chenlan; Jia, Hengke] Sichuan Univ, West China Hosp, West China Precis Med Ind Technol Inst, Dept Lab Med,Med X Ctr Mfg, Chengdu 610041, Sichuan, Peoples R China; [Shen, Chenlan; Wan, Peng; Jia, Hengke; Liu, Beizhong] Chongqing Med Univ, Yongchuan Hosp, Cent Lab, Chongqing 402160, Peoples R China; [Shen, Chenlan; Zhong, Liang; Wan, Peng; Liu, Beizhong] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Liu, BZ (通讯作者)，Chongqing Med Univ, Yongchuan Hosp, Cent Lab, Chongqing 402160, Peoples R China."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001046741300001

JCR分区：Q1

影响因子：6.2

年份：2023

卷号：1276

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Functional nucleic acid; Nanomaterials; Fusion gene; Enzyme-free biosensor

摘要："Acute promyelocytic leukemia (APL) is an acute myeloid leukemia (AML) with a specific fusion gene target, PML/RAR & alpha; fusion gene (PML/RAR & alpha;), which is formed by the translocation of chromosomes 15 and 17. Detection of PML/RAR & alpha; is the most reliable parameter for the diagnosis, treatment adjustment, efficacy evaluation, prognosis analysis and relapse prediction of APL. In this study, a novel biosensor was constructed for rapid enzyme-free detection of PML/RAR & alpha; using DNAzyme and carbon dots/cobalt oxhydroxide nanosheet complexs (CDs/CoOOH). In the detection system, the separated DNAzymes could specifically recognize and bind together by the PML/RAR & alpha; to form a complete DNAzyme for shearing hairpin probe (HP), then generated trigger, which was the first signal amplification. Then, trigger could hybridize with the capture probe (CP) anchored to streptavidin (SA) modified microplate as well as fluorescence quenching signal probe (SP@CDs/CoOOH). Finally, ascorbic acid (AA) was added to decompose CoOOH and the fluorescence of CDs was released, which was the second signal amplification. Through the dual signal amplification of DNAzyme and CDs/CoOOH, PML/ RAR & alpha; could be detected quickly and sensitively, which overcame the limitation of protein enzyme in traditional fluorescence methods, showing potential clinical application value in the diagnosis and treatment of leukemia."

基金机构：National Natural Sci-ence Foundation of China [81772280]; Project of Chongqing Science and Technology Bureau [cstc2022ycjh-bgzxm0034]; Natural Science Foundation of Sichuan Province [2023NSFSC1481]; Postdoctoral Research Foundation of West China Hospital of Sichuan University [2023HXBH122]; Sichuan University postdoctoral interdisciplinary Innovation Fund [10822041A2111]

基金资助正文："The following funds supported this study: the National Natural Sci-ence Foundation of China (No. 81772280) , the Project of Chongqing Science and Technology Bureau (No. cstc2022ycjh-bgzxm0034) , the Natural Science Foundation of Sichuan Province (2023NSFSC1481) , the Postdoctoral Research Foundation of West China Hospital of Sichuan University (2023HXBH122) and the Sichuan University postdoctoral interdisciplinary Innovation Fund (10822041A2111) ."