Electrochemical immunosensor based on hollow Pt@Cu<sub>2</sub>O as a signal label for dual-mode detection of procalcitonin
作者全名："Liu, Jie; Zhang, Zixuan; Dong, Jie; Chen, Anyi; Qiu, Jingfu; Li, Chaorui"
作者地址："[Liu, Jie; Zhang, Zixuan; Dong, Jie; Chen, Anyi; Qiu, Jingfu; Li, Chaorui] Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China"
通信作者："Li, CR (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China."
来源：TALANTA
ESI学科分类：CHEMISTRY
WOS号：WOS:001066234400001
JCR分区：Q1
影响因子：6.1
年份：2024
卷号：266
期号：
开始页：
结束页：
文献类型：Article
关键词：Rhombic dodecahedral ZIF-8; Hollow Pt@Cu2O nanocomposite; Dual-mode electrochemical immunosensor; Procalcitonin
摘要："As a reliable biomarker to evaluate the severity of sepsis, sensitive and accurate detection of procalcitonin (PCT) is essential. In this study, a dual-mode electrochemical immunosensor based on Au/ZIF-8 as substrate and Pt@Cu2O as signal label was constructed for the detection of PCT. By loading Au nanoparticles onto rhombic dodecahedral ZIF-8, the substrate (Au/ZIF-8) has large specific surface area and can immobilize antibody (Ab(1)) by Au-N bonds. Meanwhile, hollow Pt@Cu2O nanocomposite with excellent peroxidase-like activity and electrocatalytic activity were synthesized as signal label. In the process of electrochemical testing, Pt@Cu2O catalyzed the reduction of hydrogen peroxide (H2O2) and further promotes the oxidation of hydroquinone (HQ) to achieve the synergistic amplification of electrochemical signals. The proposed immunosensor detected PCT by amperometric i-t and differential pulse voltammetry (DPV) tests with a good linear response and low limit of detection (i-t: 0.70 fg/mL and DPV: 0.40 fg/mL) in the range of 10 fg/mL similar to 100 ng/mL. The immunosensor exhibited excellent sensitivity and accuracy, indicating the potential application of this method for PCT detection."
基金机构："Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0121]"
基金资助正文："This work was supported by the Natural Science Foundation of Chongqing, China (No. cstc2019jcyj-msxmX0121)."
