Multifunctional dumbbell probes-based logic circuits: microRNAs logic detection and tumor cells identification

作者全名："Liu, Xin; Li, Baiying; Liu, Qian; Zhang, Li; Zhao, Rong; Wu, Di; Wang, Luojia; Wang, Zhongzhong; Xie, Guoming; Feng, Wenli"

作者地址："[Liu, Xin; Li, Baiying; Zhang, Li; Zhao, Rong; Wu, Di; Wang, Luojia; Wang, Zhongzhong; Xie, Guoming; Feng, Wenli] Chongqing Med Univ, Dept Lab Med, Chongqing Med Lab Microfluid, Key Lab Med Diagnost,Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Liu, Xin; Li, Baiying; Zhang, Li; Zhao, Rong; Wu, Di; Wang, Luojia; Wang, Zhongzhong; Xie, Guoming; Feng, Wenli] Chongqing Med Univ, SPRi Engn Res Ctr, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Liu, Qian] Chongqing Med Univ, Dept Nucl Med, Hosp 2, Chongqing 400010, Peoples R China"

通信作者："Xie, GM; Feng, WL (通讯作者)，Chongqing Med Univ, Dept Lab Med, Chongqing Med Lab Microfluid, Key Lab Med Diagnost,Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China.; Xie, GM; Feng, WL (通讯作者)，Chongqing Med Univ, SPRi Engn Res Ctr, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001093327800001

JCR分区：Q1

影响因子：6.2

年份：2023

卷号：1280

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Dumbbell probes; Logic gates; Primer exchange reaction; MicroRNAs; Cells identification

摘要："Background: The powerful logic processing capability of DNA logic circuits over multiple input signals perfectly meets the demands of multi-biomarker-based clinical diagnostics. As important biomarkers for cancer diagnosis and treatment, the orthogonal differential expression of microRNAs (miRNAs) in different diseases and different cancer cells makes the precise logical detection of multiple miRNAs particularly critical. Results: Therefore, we constructed two fundamental ""AND"" and ""OR"" logic gates and one ""AND-OR"" logic gate on the basis of our proposed multifunctional dumbbell probes. These logic gates allowed for the logical profiling of multiple cancer-associated miRNAs. In addition, by making simple adjustments to the functional modules of multifunctional dumbbell probes, the three logic gates we proposed could be easily transformed without the use of sophisticated probe design. Remarkably, these logic gates, in particular the ""AND-OR"" logic gate, were able to compute several miRNAs simultaneously, demonstrating excellent cell identification capabilities. Significance: Overall, this work provided a new idea for accurately distinguishing multiple cell types and showed great application prospects."

基金机构：National key R & D Program of China [2022YFC2603800]; National Natural Science Foundation of China; [82172369]

基金资助正文：This work was financially supported by the National key R & D Program of China (2022YFC2603800) and National Natural Science Foundation of China (82172369) .