Cordycepin delays postovulatory aging of oocytes through inhibition of maternal mRNAs degradation via DCP1A polyadenylation suppression

作者全名："Li, Chong; Zhu, Ling; Liu, Jun-Xia; Guo, Jing; Xie, Juan; Shi, Chun-Meng; Sun, Qing-Yuan; Huang, Guo-Ning; Li, Jing-Yu"

作者地址："[Li, Chong; Zhu, Ling; Liu, Jun-Xia; Xie, Juan; Huang, Guo-Ning; Li, Jing-Yu] Women & Childrens Hosp Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Chongqing, Peoples R China; [Li, Chong; Zhu, Ling; Liu, Jun-Xia; Xie, Juan; Huang, Guo-Ning; Li, Jing-Yu] Chongqing Clin Res Ctr Reprod Med, Chongqing Hlth Ctr Women & Children, Chongqing, Peoples R China; [Guo, Jing] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun, Peoples R China; [Shi, Chun-Meng] Third Mil Med Univ, Inst Rocket Force Med, State Key Lab Trauma Burns & Combined Injury, Chongqing, Peoples R China; [Sun, Qing-Yuan] Guangdong Second Prov Gen Hosp, Reprod Med Ctr, Guangzhou Key Lab Metab Dis & Reprod Hlth, Guangzhou 510310, Peoples R China"

通信作者："Huang, GN; Li, JY (通讯作者)，Women & Childrens Hosp Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Chongqing, Peoples R China.; Huang, GN; Li, JY (通讯作者)，Chongqing Clin Res Ctr Reprod Med, Chongqing Hlth Ctr Women & Children, Chongqing, Peoples R China.; Shi, CM (通讯作者)，Third Mil Med Univ, Inst Rocket Force Med, State Key Lab Trauma Burns & Combined Injury, Chongqing, Peoples R China.; Sun, QY (通讯作者)，Guangdong Second Prov Gen Hosp, Reprod Med Ctr, Guangzhou Key Lab Metab Dis & Reprod Hlth, Guangzhou 510310, Peoples R China."

来源：CELLULAR AND MOLECULAR LIFE SCIENCES

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001106468300002

JCR分区：Q2

影响因子：8

年份：2023

卷号：80

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：Postovulatory aging; Maternal mRNAs; Cordycepin; DCP1A

摘要："Postovulatory aging leads to the decline in oocyte quality and subsequent impairment of embryonic development, thereby reducing the success rate of assisted reproductive technology (ART). Potential preventative strategies preventing oocytes from aging and the associated underlying mechanisms warrant investigation. In this study, we identified that cordycepin, a natural nucleoside analogue, promoted the quality of oocytes aging in vitro, as indicated by reduced oocyte fragmentation, improved spindle/chromosomes morphology and mitochondrial function, as well as increased embryonic developmental competence. Proteomic and RNA sequencing analyses revealed that cordycepin inhibited the degradation of several crucial maternal proteins and mRNAs caused by aging. Strikingly, cordycepin was found to suppress the elevation of DCP1A protein by inhibiting polyadenylation during postovulatory aging, consequently impeding the decapping of maternal mRNAs. In humans, the increased degradation of DCP1A and total mRNA during postovulatory aging was also inhibited by cordycepin. Collectively, our findings demonstrate that cordycepin prevents postovulatory aging of mammalian oocytes by inhibition of maternal mRNAs degradation via suppressing polyadenylation of DCP1A mRNA, thereby promoting oocyte developmental competence."

基金机构："Natural Science Foundation of Chongqing Province [cstc2021jcyj-msxmX0823, CSTB2022NSCQ-MSX0875]; Special research project of the Chongqing Health Center for Women and Children [2020YJMS03]"

基金资助正文："The work was supported by the Natural Science Foundation of Chongqing Province (cstc2021jcyj-msxmX0823 and CSTB2022NSCQ-MSX0875), the special research project of the Chongqing Health Center for Women and Children (2020YJMS03)."