A buffering fluorogenic probe for real-time lysosomal pH monitoring

作者全名："Zhang, Yantao; Liu, Qian; Tian, Tao; Xu, Chunhua; Yang, Pengli; Ma, Lianju; Hou, Yi; Zhou, Hui; Gan, Yongjun"

作者地址："[Zhang, Yantao; Liu, Qian; Tian, Tao; Xu, Chunhua; Yang, Pengli; Ma, Lianju; Hou, Yi; Gan, Yongjun] Chongqing Med Univ, Lab Pharm & Chem, Lab Teaching & Management Ctr, Chongqing 400016, Peoples R China; [Zhang, Yantao; Tian, Tao; Xu, Chunhua; Yang, Pengli; Zhou, Hui] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China"

通信作者："Gan, YJ (通讯作者)，Chongqing Med Univ, Lab Pharm & Chem, Lab Teaching & Management Ctr, Chongqing 400016, Peoples R China."

来源：SENSORS AND ACTUATORS B-CHEMICAL

ESI学科分类：CHEMISTRY

WOS号：WOS:001112219600001

JCR分区：Q1

影响因子：8.4

年份：2024

卷号：399

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：PH probe; Naphthalimide derivative; Buffer strategy; Photo-induced electron transfer

摘要："Real-time monitoring of lysosomal pH is critical for understanding lysosome-related biological processes and diseases, but few lysosome probes can achieve this response effectively. Therefore, it is essential to develop pHsensitive probes with better performance. In this study, we designed and synthesized a lysosome-targeting ""buffering fluorogenic probe"" (Lyso-BFP) by introducing a salicylamide group to naphthalimide for buffering strategy and a hydroxyethyl piperazine unit to target lysosome as well as specific pH response. Lyso-BFP demonstrated excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells, thanks to its well-suit pKa. Density functional theory calculation revealed that Lyso-BFP's excellent pH sensing performance resulted from the inhibition of the photo-induced electron transfer (PET) process from hydroxyethyl piperazine to the naphthalimide moiety after the piperazine ring's protonation at low pH. Remarkably, Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy. With its superior pH selectivity and realtime pH monitoring capability, Lyso-BFP holds great potential in the diagnosis of lysosome-related disease, and the design concept of this fluorogenic probe provides a new perspective for real-time tracking of cell organelles."

基金机构："Natural Science Foundation of Chongqing [cstc2020jcyjmsxmX0622]; Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN201900405]; Program for Youth Innovation in Future Medicine of Chongqing Medical University [W0105]; Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University [202127]; Project of Tutorial System of Medical Undergraduate in Lab Teaching & Management Center in Chongqing Medical University [LTMCMTS202120, LTMCMTS202123]"

基金资助正文："The work was supported by Natural Science Foundation of Chongqing (cstc2020jcyjmsxmX0622) , the Project Supported by Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN201900405) , Program for Youth Innovation in Future Medicine of Chongqing Medical University (W0105) , The Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University (202127) , Project of Tutorial System of Medical Undergraduate in Lab Teaching & Management Center in Chongqing Medical University (LTMCMTS202120, LTMCMTS202123) ."