IL-37 counteracts inflammatory injury in the temporomandibular joint via the intracellular pathway

作者全名："Li, Jun; Peng, Sisi; Yan, Ying; Yan, Shan; Cao, Xin; Li, Yong; Zhu, Luying; Xu, Jie"

作者地址："[Li, Jun; Peng, Sisi; Yan, Ying; Yan, Shan; Cao, Xin; Li, Yong; Zhu, Luying; Xu, Jie] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Li, Jun; Peng, Sisi; Yan, Ying; Yan, Shan; Cao, Xin; Li, Yong; Zhu, Luying; Xu, Jie] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Li, Jun; Peng, Sisi; Yan, Ying; Yan, Shan; Cao, Xin; Li, Yong; Zhu, Luying; Xu, Jie] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Li, Jun] Chongqing Emergency Med Ctr, Chongqing, Peoples R China; [Li, Jun] Chongqing Univ, Cent Hosp, Chongqing, Peoples R China"

通信作者："Zhu, LY; Xu, J (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China.; Zhu, LY; Xu, J (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Zhu, LY; Xu, J (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."

来源：FRONTIERS IN PHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001112628300001

JCR分区：Q1

影响因子：5.6

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：intracellular IL-37; inflammation; temporomandibular joint; synovitis; osteoarthritis

摘要："Background: The temporomandibular joint is often afflicted by osteoarthritis (TMJOA), causing pain and dysfunction, which is particularly prevalent in the elderly population. IL-37 is effective in avoiding excessive inflammatory damage to the organism. This article investigates the role and mechanism of intracellular IL-37 in TMJOA.Methods: Enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, Western blotting, Senescence-associated beta-galactosidase staining, immunofluorescence, and lentivirus were performed to elucidate the underlying mechanism.Results: The results confirmed that IL-37 in synovial cells decreased with aging. Inflammatory stimulus elevated intracellular IL-37 in synoviocytes, while lentiviral knockdown of IL-37 resulted in more inflammatory factor production. Dynamic changes of IL-37 were observed in the nucleus and supernatant. In addition, Caspease-1 inhibitor hindered intracellular IL-37 maturation, and Smad3 inhibitor caused the loss of nuclear translocation of mature IL-37. Transfection of synovial cells with IL-37-expressing lentivirus resulted in relief not only of synovitis but also of the cartilage damage and inflammation caused by synovitis.Conclusion: This study provides new insights into the intracellular anti-inflammatory mechanism of IL-37. It also confirms that IL-37 decreases with cellular senescence and that increasing intracellular IL-37 can effectively treat synovitis and synovitis-induced inflammatory damage to cartilage."

基金机构：National Natural Science Foundation of China [81800999]; Chongqing Health Commission and Science and Technology Bureau Foundation [2023ZDXM019]

基金资助正文："This work was supported by the National Natural Science Foundation of China (81800999 to JX), the Chongqing Health Commission and Science and Technology Bureau Foundation (2023ZDXM019 to JX)."