Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice

作者全名："Tan, Weilin; Ma, Jun; Fu, Jiayuanyuan; Wu, Biying; Zhu, Ziyu; Huang, Xuekang; Du, Mengran; Wu, Chenrui; Balawi, Ehab; Zhou, Qiang; Zhang, Jie; Liao, Zhengbu"

作者地址："[Tan, Weilin; Ma, Jun; Fu, Jiayuanyuan; Wu, Biying; Zhu, Ziyu; Huang, Xuekang; Du, Mengran; Wu, Chenrui; Balawi, Ehab; Zhou, Qiang; Zhang, Jie; Liao, Zhengbu] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, Chongqing, Peoples R China"

通信作者："Liao, ZB (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, Chongqing, Peoples R China."

来源：NEURAL REGENERATION RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:001116418500028

JCR分区：Q2

影响因子：6.1

年份：2024

卷号：19

期号：1

开始页：171

结束页：179

文献类型：Article

关键词：axon guidance; bioinformatics analysis; competing endogenous RNA; erythropoietin; Gene Ontology; Kyoto Encyclopedia of Genes and Genomes; non-coding RNA; RNA sequencing; transcriptomics; traumatic brain injury

摘要："Recent studies have found that erythropoietin promotes the recovery of neurological function after traumatic brain injury. However, the precise mechanism of action remains unclear. In this study, we induced moderate traumatic brain injury in mice by intraperitoneal injection of erythropoietin for 3 consecutive days. RNA sequencing detected a total of 4065 differentially expressed RNAs, including 1059 mRNAs, 92 microRNAs, 799 long non-coding RNAs, and 2115 circular RNAs. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses revealed that the coding and non-coding RNAs that were differentially expressed after traumatic brain injury and treatment with erythropoietin play roles in the axon guidance pathway, Wnt pathway, and MAPK pathway. Constructing competing endogenous RNA networks showed that regulatory relationship between the differentially expressed non-coding RNAs and mRNAs. Because the axon guidance pathway was repeatedly enriched, the expression of Wnt5a and Ephb6, key factors in the axonal guidance pathway, was assessed. Ephb6 expression decreased and Wnt5a expression increased after traumatic brain injury, and these effects were reversed by treatment with erythropoietin. These findings suggest that erythropoietin can promote recovery of nerve function after traumatic brain injury through the axon guidance pathway."

基金机构："National Natural Science Foundation of China [81771355]; Natural Science Foundation of Chongqing Science and Technology Bureau [CSTC2015jcyjA10096, cstc2021jcyj-msxmX0262]"

基金资助正文："This study was supported by the National Natural Science Foundation of China, No. 81771355; and the Natural Science Foundation of Chongqing Science and Technology Bureau, Nos. CSTC2015jcyjA10096, cstc2021jcyj-msxmX0262 (all to ZL)."