Ultrasensitive detection of genetic variation based on dual signal amplification assisted by isothermal amplification and cobalt oxyhydroxide nanosheets/quantum dots

作者全名："Shen, Chenlan; Wang, Tong; Yang, Ke; Zhong, Liang; Liu, Beizhong"

作者地址："[Shen, Chenlan] Sichuan Univ, West China Hosp, Dept Lab Med, Chengdu 610041, Sichuan, Peoples R China; [Shen, Chenlan; Liu, Beizhong] Chongqing Med Univ, Cent Lab, Yongchuan Hosp, Chongqing 402160, Peoples R China; [Wang, Tong] Fourth Peoples Hosp Chengdu, Clin Lab, Chengdu 610036, Sichuan, Peoples R China; [Yang, Ke] Chengdu Shangjin Nanfu Hosp, Dept Lab Med, Chengdu 611743, Sichuan, Peoples R China; [Zhong, Liang; Liu, Beizhong] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Liu, BZ (通讯作者)，Chongqing Med Univ, Cent Lab, Yongchuan Hosp, Chongqing 402160, Peoples R China.; Liu, BZ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：MICROCHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001117724000001

JCR分区：Q1

影响因子：5.7

年份：2024

卷号：191

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：PML/RAR alpha fusion gene; Tri-HT SDA; CoOOH/QD; Genetic variation detection; Fluorescence assay

摘要："PML/RAR alpha fusion gene (P/R) is the characteristic signature genetic variation of acute promyelocytic leukemia (APL). Here, by integrating triple-stranded DNA hybridization-triggered strand displacement amplification (tri-HT SDA) and cobalt oxyhydroxide nanosheets/quantum dots (CoOOH/QD)-based amplification, we constructed a novel biosensor of easy-operating, time-saving and high sensitivity for detecting P/R to meet clinical needs. Owing to the specific recognition and efficient amplification of tri-HT SDA as well as impressive anti-interference and considerable amplification of CoOOH/QD, this biosensor demonstrated a wide dynamic range (10 fM to 10 nM) with a low limit of detection (5.50 fM) in P/R detection. Additionally, this biosensor could detect P/R spiked into human serum with good recoveries and relative standard deviation (RSD), thus potentially exhibiting ultrasensitive and specific nuclear acid sequence detection ability in clinical diagnosis owing to combing isothermal amplification and nanomaterials."

基金机构：National Natural Science Foundation of China

基金资助正文：No Statement Available