Antigen retrieval assisted electrochemiluminescence imaging of surface antigens at single tissue section

作者全名："Fu, Chengfeng; Han, Dongni; Jiang, Dechen; Jiang, Depeng"

作者地址："[Fu, Chengfeng; Jiang, Depeng] Chongqing Med Univ, Affiliated Hosp 2, Dept Resp Med, Chongqing 400010, Peoples R China; [Fu, Chengfeng; Han, Dongni; Jiang, Dechen] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China; [Fu, Chengfeng] Second Peoples Hosp Banan Dist, Dept Urol, Chongqing, Peoples R China"

通信作者："Jiang, DP (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Resp Med, Chongqing 400010, Peoples R China.; Jiang, DC (通讯作者)，Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China."

来源：ELECTROCHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001125926700001

JCR分区：Q1

影响因子：6.6

年份：2024

卷号：474

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Electrochemiluminescence imaging; Antigen retrieval; Surface antigens; Single tissue section; CEA

摘要："Previously, our group has established an electrochemiluminescence (ECL) molecular imaging method to detect carcinoembryonic antigen (CEA) at single tissue sections of carcinoma patients. However, the tissue fixation blocks the binding sites of surface antigens affecting the antigen-antibody interaction and the resultant visualization sensitivity. Herein, we report an antigen retrieval assisted ECL imaging strategy that utilizes heat-induced epitope retrieval (HIER) to re-expose the binding sites of the antigens for more efficient interaction between surface antigen and ruthenium-labeled antibody. With more ruthenium complexes tagged with surface antigens, the ECL intensity at the tissue sections increases significantly which could more accurately reflect the amount and the distribution of surface CEA. Accordingly, the different distribution of CEA at the carcinoma and paracancerous regions of tissue sections from two patients with lung adenocarcinoma (LUAD) is visualized, which could not be easily distinguished without the HIER treatment. Eventually, the combination of antigen retrieval and ECL molecular imaging could improve the visualization sensitivity at single tissue section and minimize the false negative phenomenon, which will greatly advance the development of ECL-based tissue imaging for future application in pathological diagnosis."

基金机构：Chongqing Natural Science Foundation of China [cstc2022ycjh-bgzxm0051]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University

基金资助正文："This work is supported by the Chongqing Natural Science Foundation of China (cstc2022ycjh-bgzxm0051) , and Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University."