Immunomultiple PCR-based electrochemical and lateral flow strategy for the simultaneous detection of liver cancer tumor markers

作者全名："Hu, Juan; Ren, Wubo; Liu, Han; Xie, Cong; Li, Dandan; Yang, Liping; Liao, Fangli; Jiang, Linshan; Pu, Qinli; Chen, Weixian"

作者地址："[Hu, Juan; Ren, Wubo; Li, Dandan; Yang, Liping; Liao, Fangli; Jiang, Linshan; Pu, Qinli; Chen, Weixian] Chongqing Med Univ, Affiliated Hosp 2, Dept Lab Med, 74 Linjiang Rd, Chongqing 400010, Peoples R China; [Hu, Juan] Chongqing Med & Pharmaceut Coll, Affiliated Hosp 1, Expt Res Ctr, Chongqing 400060, Peoples R China; [Liu, Han] Jiulongpo Dist Peoples Hosp, Dept Neurol, Chongqing 400050, Peoples R China; [Xie, Cong] Peoples Hosp Chongqing Liang Jiang New Area, Dept Clin Lab, Chongqing 401122, Peoples R China"

通信作者："Pu, QL; Chen, WX (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Lab Med, 74 Linjiang Rd, Chongqing 400010, Peoples R China."

来源：MICROCHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001126259500003

JCR分区：Q1

影响因子：5.7

年份：2024

卷号：191

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Antigen capture polymerase chain reaction; Tumor biomarkers; Liver cancer; Lateral flow assay; Electrochemical biosensor; Square wave voltammetry

摘要："The current use of the single serum biomarker alpha-fetoprotein (AFP) in clinical practice has limitations in terms of specificity and sensitivity. We propose a strategy that combines antigen capture polymerase chain reaction (AC-PCR), lateral flow assay (LFA), and electrochemical biosensors to detect both AFP and circulating tumor cells (CTCs) in liver cancer serum. First, we used the AC-PCR technique to achieve target separation, purification, signal conversion, and amplification, eliminating target heterogeneity. Then, we achieved rapid results through the LFA and electrochemical biosensor platforms. As a result, the proposed assay has limits of 5 cells/mL for CTCs and 5 mu g/L for AFP. The proposed method was applied effectively to simulated blood samples. This method has the potential to play a role in early liver cancer and provide a potential application for the diagnosis and precision treatment of liver cancer."

基金机构：National Natural Science Foundation of China; Department of Laboratory Medicine at The Second Affiliated Hospital of Chongqing Medical University

基金资助正文：We express our gratitude to the Department of Laboratory Medicine at The Second Affiliated Hospital of Chongqing Medical University for their generous assistance and support during this project.