Modulating Lineage Specification in Stem Cell Differentiation via Bioelectrical Stimulation Intensity Matching

作者全名："Zhang, Fengyi; Yan, Xiangyu; Wu, Muyao; Chen, Yumin; Zhao, Han; Zhang, Chenguang; Dang, Pengrui; Wei, Ling; Zhu, Fangyu; Chen, Ying; Song, Jinlin; Li, Zhihong; Deng, Xuliang; Liu, Wenwen"

作者地址："[Zhang, Fengyi; Li, Zhihong] Cent South Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410011, Peoples R China; [Zhang, Fengyi; Li, Zhihong] Cent South Univ, Xiangya Hosp 2, Hunan Key Lab Tumor Models & Individualized Med, Changsha 410011, Peoples R China; [Yan, Xiangyu] Cent South Univ, Key Lab Powder Met, Changsha 410083, Peoples R China; [Wu, Muyao] Hunan Normal Univ, Key Lab Translat Canc Stem Cell Res, Changsha 410081, Peoples R China; [Chen, Yumin; Zhao, Han; Zhu, Fangyu; Chen, Ying; Deng, Xuliang; Liu, Wenwen] Peking Univ, Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China; [Zhang, Chenguang] Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Guangzhou 510055, Peoples R China; [Dang, Pengrui] China Med Univ, Sch & Hosp Stomatol, VIP Dept, Liaoning Prov Key Lab Oral Dis, Shenyang 110002, Peoples R China; [Wei, Ling] Peking Univ, Sch & Hosp Stomatol, Clin Div 3, Beijing 100081, Peoples R China; [Song, Jinlin] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China"

通信作者："Li, ZH (通讯作者)，Cent South Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410011, Peoples R China.; Li, ZH (通讯作者)，Cent South Univ, Xiangya Hosp 2, Hunan Key Lab Tumor Models & Individualized Med, Changsha 410011, Peoples R China.; Deng, XL; Liu, WW (通讯作者)，Peking Univ, Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China."

来源：ADVANCED MATERIALS INTERFACES

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001126423300001

JCR分区：Q2

影响因子：5.4

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：bioelectrical stimulation; neurogenic differentiation; osteogenic differentiation; stem-cell-fate commitment; surface potential

摘要："Development and regeneration in biological tissues are fundamentally affected by stem-cell-fate commitment. Bioelectricity is heterogeneous between different tissues and crucially regulates cell behaviors, including cell differentiation. However, the effects of heterogeneous bioelectricity on stem-cell differentiation remain poorly understood. Herein, it is shown that providing stem cells with electrical stimulation matching the endogenous membrane potentials of cells derived from different tissues (osteogenic-related: -55.05 +/- 4.22 mV, neurogenic-related: -84.8 +/- 7.48 mV) can induce their osteogenic or neurogenic lineage commitment. Molecular dynamics simulations indicated that the osteogenic-related surface potential favors the adsorption of fibronectin, while the neurogenic-related surface potential enhances the adsorption of FGF-2. These different protein adsorptions trigger either downstream Wnt or Erk signaling, which direct stem-cell differentiation. Surface-potential-mediated lineage-specification of stem cells using bioelectrical intensity has enormous potential application value in tissue regenerative therapy. Matching the bioelectrical stimulation intensity with the endogenous membrane potentials of cells derived from different tissues (osteogenic: -55.05 +/- 4.22 mV, neurogenic: -84.8 +/- 7.48 mV) can modulate osteogenic or neurogenic lineage commitment of stem cells by regulating protein adsorption and the activation of Wnt or Erk signaling. This study provides a theoretical basis for understanding bioelectric microenvironment modulated organogenesis.image"

基金机构："China Postdoctoral Science Foundation; Provincial Natural Science Foundation of Hunan [2022JJ40725]; National Natural Science Foundation of China [81600905]; Peking University Medicine Fund of Fostering Young Scholars' Scientific & Technological Innovation; Fundamental Research Funds for the Central Universities [BMU2018PY005]; National Key Research and Development Program of China [2018YFE0194400, 2022YFC2504200]; High-Performance Computing Center of Central South University; [2021M703634]"

基金资助正文："The authors thank the China Postdoctoral Science Foundation (2021M703634), the Provincial Natural Science Foundation of Hunan (2022JJ40725), the National Natural Science Foundation of China (Grant No. U22A20314), the National Natural Science Foundation of China (81600905), the Peking University Medicine Fund of Fostering Young Scholars' Scientific & Technological Innovation supported by the Fundamental Research Funds for the Central Universities (BMU2018PY005), the National Key Research and Development Program of China (2018YFE0194400, 2022YFC2504200) for supporting this work. This work was supported in part by the High-Performance Computing Center of Central South University. Figures 3a, 4a, 5a, and 6a, and Figure S1 (Supporting Information) were created with BioRender.com. Human dental pulp stem cells (DPSCs) were provided by ORAL STEM CELL BANK run by Beijing Tason Biotech Co., Ltd."