A novel lung cancer stem cell extracellular vesicles lncRNA ROLLCSC modulate non-stemness cancer cell plasticity through miR-5623-3p and miR-217-5p targeting lipid metabolism

作者全名："Zhang, Yu-Han; Chen, Yan; Shi, Lei; Han, Xue; Xie, Jia-Cheng; Chen, Yuting; Xiang, Meng; Li, Bo-Wen; Li, Jie; Xing, Rosie; Wang, Jian-Yu"

作者地址："[Zhang, Yu-Han; Chen, Yan; Shi, Lei; Xie, Jia-Cheng; Chen, Yuting; Xiang, Meng; Li, Bo-Wen; Xing, Rosie] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Zhang, Yu-Han; Chen, Yan; Shi, Lei; Xie, Jia-Cheng; Chen, Yuting; Xiang, Meng; Li, Bo-Wen; Xing, Rosie] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China; [Han, Xue; Li, Jie; Wang, Jian-Yu] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China; [Xing, Rosie; Wang, Jian-Yu] Yi Xue Yuan Rd, Chongqing 400016, Peoples R China"

通信作者："Xing, RS; Wang, JY (通讯作者)，Yi Xue Yuan Rd, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001132155600001

JCR分区：Q1

影响因子：8.2

年份：2024

卷号：256

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Long noncoding RNA; Extracellular vesicles; Cancer stem cells; Lipid metabolism

摘要："Background: The high mortality rate of lung cancer is largely attributed to metastasis. Lung cancer stem cells (CSC) are conducive to cancer heterogeneity. Long noncoding RNAs are known to participate in various biological processes regulating the development of lung cancer. However, characterization of the role and mechanisms of lncRNA in lung cancer metastasis remains a challenge.Results: We demonstrate that ROLLCSC, a highly expressed lncRNA in LLC-SDs, promotes the metastasis of the low metastatic LLCs both in vitro and in vivo. ROLLCSC can be transferred from LLC-SD to LLC through encapsulation in extracellular vesicles (EVs), ultimately leading to the enhancement of the metastatic phenotype of LLCs. Mechanistically, we demonstrate that the pro-metastatic activity of ROLLCSC is achieved through its function as a competing endogenous RNA (ceRNA) of miR-5623-3p and miR-217-5p to stimulate lipid metabolism.Conclusion: In this study, we have characterized ROLLCSC, a novel lncRNA, as a pivotal regulator in the metastasis of lung cancer, highlighting its potential as a therapeutic target. Specifically, we show that ROLLCSC is encapsulated by the EVs of LLC-SDs and transmitted to the LLCs, where it acts as a ceRNA of miR-5623-3p and miR-217-5p to stimulate lipid metabolism and ultimately augments metastatic colonization of LLCs."

基金机构："National Natural Science Fund [82073277, 82173247]; Science and Technology Project Affiliated to the Education Department of Chongqing [KJQN202100404]; Project of Chongqing Natural Science Foundation Innovation and Development Fund (Municipal Education Commission) [CSTB2022NS CQ-LZX0023]"

基金资助正文："This work was supported by the National Natural Science Fund (Grant Nos. 82073277 and 82173247) , the Science and Technology Project Affiliated to the Education Department of Chongqing (Grant No. KJQN202100404) , and Project of Chongqing Natural Science Foundation Innovation and Development Fund (Municipal Education Commission) (Grant No. CSTB2022NS CQ-LZX0023) ."