Engineering of a Self-Regulatory Bidirectional DNA Assembly Circuit for Amplified MicroRNA Imaging

作者全名："Gong, Xue; Zhang, Jiajia; Zhang, Pu; Jiang, Yuqian; Hu, Lianzhe; Jiang, Zhongwei; Wang, Fuan; Wang, Yi"

作者地址："[Gong, Xue; Zhang, Jiajia; Hu, Lianzhe; Jiang, Zhongwei; Wang, Yi] Chongqing Normal Univ, Coll Chem, Engn Res Ctr Biotechnol Act Subst, Minist Educ,Chongqing Key Lab Green Synth & Applic, Chongqing 401331, Peoples R China; [Zhang, Pu] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Jiang, Yuqian; Wang, Fuan] Wuhan Univ, Res Inst Shenzhen, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China"

通信作者："Gong, X; Wang, Y (通讯作者)，Chongqing Normal Univ, Coll Chem, Engn Res Ctr Biotechnol Act Subst, Minist Educ,Chongqing Key Lab Green Synth & Applic, Chongqing 401331, Peoples R China.; Wang, F (通讯作者)，Wuhan Univ, Res Inst Shenzhen, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China."

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:001132961000001

JCR分区：Q1

影响因子：7.4

年份：2023

卷号：95

期号：51

开始页：18731

结束页：18738

文献类型：Article

关键词：　

摘要："The engineering of catalytic hybridization DNA circuits represents versatile ways to orchestrate a complex flux of molecular information at the nanoscale, with potential applications in DNA-encoded biosensing, drug discovery, and therapeutics. However, the diffusive escape of intermediates and unintentional binding interactions remain an unsolved challenge. Herein, we developed a compact, yet efficient, self-regulatory assembly circuit (SAC) for achieving robust microRNA (miRNA) imaging in live cells through DNA-templated guaranteed catalytic hybridization. By integrating the toehold strand with a preblocked palindromic fragment in the stem domain, the proposed miniature SAC system allows the reactant-to-template-controlled proximal hybridization, thus facilitating the bidirectional-sustained assembly and the localization-intensified signal amplification without undesired crosstalk. With condensed components and low reactant complexity, the SAC amplifier realized high-contrast intracellular miRNA imaging. We anticipate that this simple and template-controlled design can enrich the clinical diagnosis and prognosis toolbox."

基金机构："Natural Science Foundation of Chongqing [22204012, 81972020, 22074112]; National Natural Science Foundation of China [CSTB2022NSCQ-MSX0466, cstc2021ycjh-bgzxm0113]; Natural Science Foundation of Chongqing [CXQT21015]; Chongqing Innovation Research Group Project [KJQN202200559]; Science and Technology Research Project of Chongqing Municipal Education Commission [JCYJ20220530140609021, JCYJ20230807090210020]; Shenzhen Science and Technology Program [22XLB010]; Startup Fund of Chongqing Normal University; Chongqing Youth Expert Studio"

基金资助正文："This work was supported by the National Natural Science Foundation of China (22204012, 81972020, and 22074112), the Natural Science Foundation of Chongqing (CSTB2022NSCQ-MSX0466 and cstc2021ycjh-bgzxm0113), the Chongqing Innovation Research Group Project (CXQT21015), the Science and Technology Research Project of Chongqing Municipal Education Commission (KJQN202200559), Shenzhen Science and Technology Program (JCYJ20220530140609021 and JCYJ20230807090210020), and the Startup Fund of Chongqing Normal University (22XLB010). Y.W. was also sponsored by the Chongqing Youth Expert Studio."