STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells

作者全名："Wang, Xian; Han, Chao; Yang, Di; Zhou, Jian; Dong, Hui; Wei, Zhiyuan; Xu, Shuai; Xu, Chen; Zhang, Yiwei; Sun, Yi; Ni, Bing; Guo, Sheng; Zhang, Jingbo; Zhao, Tingting; Chen, Xiangmei; Luo, Jie; Wu, Yuzhang; Tian, Yi"

作者地址："[Wang, Xian; Han, Chao; Yang, Di; Zhou, Jian; Dong, Hui; Xu, Chen; Zhang, Yiwei; Guo, Sheng; Wu, Yuzhang; Tian, Yi] Third Mil Med Univ, Army Med Univ, Inst Immunol, Chongqing 400038, Peoples R China; [Wang, Xian] Qingdao Univ, Dept Immunol, Med Coll, Qingdao 266071, Shandong, Peoples R China; [Wei, Zhiyuan; Sun, Yi; Luo, Jie] Third Mil Med Univ, Army Med Univ, Affiliated Hosp 1, Chongqing 400038, Peoples R China; [Xu, Shuai; Zhang, Jingbo] Third Mil Med Univ, Affiliated Hosp 2, Army Med Univ, Chongqing 400037, Peoples R China; [Ni, Bing] Third Mil Med Univ, Army Med Univ, Dept Pathophysiol, Chongqing 400038, Peoples R China; [Zhao, Tingting; Wu, Yuzhang] Chongqing Int Inst Immunol, Chongqing 400030, Peoples R China; [Chen, Xiangmei] Chinese PLA Inst Nephrol, Chinese PLA Gen Hosp, Dept Nephrol, State Key Lab Kidney Dis,Natl Clin Res Ctr Kidney, Beijing 100853, Peoples R China"

通信作者："Wu, YZ; Tian, Y (通讯作者)，Third Mil Med Univ, Army Med Univ, Inst Immunol, Chongqing 400038, Peoples R China.; Wu, YZ (通讯作者)，Chongqing Int Inst Immunol, Chongqing 400030, Peoples R China."

来源：COMMUNICATIONS BIOLOGY

ESI学科分类：　

WOS号：WOS:001136085900005

JCR分区：Q1

影响因子：5.9

年份：2024

卷号：7

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Retinoid-related orphan receptor gamma t (ROR gamma t) is the lineage-specific transcription factor for T helper 17 (Th17) cells. Our previous study demonstrated that STAT3 likely participates in the activation of RORCE2 (a novel enhancer of the ROR gamma t gene) in Th17 cells. However, the detailed mechanism is still unclear. Here, we demonstrate that both STAT3 and SOX-5 mediate the enhancer activity of RORCE2 in vitro. Deletion of the STAT3 binding site (STAT3-BS) in RORCE2 impaired ROR gamma t expression and Th17 differentiation, resulting in reduced severity of experimental autoimmune encephalomyelitis (EAE). Mechanistically, STAT3 and SOX-5 bind the RORCE2 region and recruit the chromatin remodeling factor BRG1 to remodel the nucleosomes positioned at this region. Collectively, our data suggest that STAT3 and SOX-5 mediate the differentiation of Th17 cells through the induction of BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells. STAT3 and SOX-5 may mediate Th17 cell differentiation through the induction of BRG1-mediated chromatin remodeling of RORCE2."

基金机构："National Natural Science Foundation of China (National Science Foundation of China) [32100708]; Young Scientists Fund of the National Natural Science Foundation of China [32170887, 31770986]; General Program of the National Natural Science Foundation of China [32141005]; Special Program of the National Natural Science Foundation of China [92269110]; Major Research Plan of the National Natural Science Foundation of China [cstc2022ycjh-bgzxm0020]; Chongqing Talent Program of China [cstc2021jxjl130036]; Special fund for performance incentive guidance of research institutions in Chongqing [2022YJC01]; Chongqing International Institute for Immunology Project"

基金资助正文："This work was supported by grants from the Young Scientists Fund of the National Natural Science Foundation of China (No. 32100708), the General Program of the National Natural Science Foundation of China (No. 32170887 and 31770986), the Special Program of the National Natural Science Foundation of China (No. 32141005), and the Major Research Plan of the National Natural Science Foundation of China (No. 92269110), as well as the Chongqing Talent Program of China (cstc2022ycjh-bgzxm0020), Special fund for performance incentive guidance of research institutions in Chongqing (cstc2021jxjl130036) and Chongqing International Institute for Immunology Project (2022YJC01). The funders had no role in the study design, data analyses, or the decision to publish."