Panax notoginseng saponin R1 improves glucocorticoid-inhibited airway epithelium repair via glucocorticoid receptor β

作者全名："Yang, Lili; Xu, Maozhu; Zhang, Linghuan; Zhang, Mingxiang; Wu, Wenjie; Luo, Zhengxiu; Tian, Daiyin; Fu, Zhou; Zou, Wenjing"

作者地址："[Yang, Lili; Xu, Maozhu; Zhang, Linghuan; Zhang, Mingxiang; Wu, Wenjie; Luo, Zhengxiu; Tian, Daiyin; Fu, Zhou; Zou, Wenjing] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, Dept Resp Med,Childrens Hosp,Minist Educ, Chongqing, Peoples R China; [Zhang, Linghuan; Zhang, Mingxiang; Luo, Zhengxiu; Tian, Daiyin; Fu, Zhou; Zou, Wenjing] Chongqing Key Lab Pediat, Chongqing, Peoples R China; [Wu, Wenjie] Chongqing YouYouBaoBei Women & Childrens Hosp, Dept Pediat, Chongqing, Peoples R China; [Fu, Zhou; Zou, Wenjing] Chongqing Med Univ, Childrens Hosp, Dept Resp Med, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China"

通信作者："Fu, Z; Zou, WJ (通讯作者)，Chongqing Med Univ, Childrens Hosp, Dept Resp Med, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China."

来源：INTERNATIONAL IMMUNOPHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001139386500001

JCR分区：Q1

影响因子：5.6

年份：2024

卷号：127

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Asthma; Panax notoginseng saponin R1; Dexamethasone; GR beta; MKP-1; Erk1/2

摘要："Background: Panax notoginseng saponin R1(PNS-R1), derived from Panax notoginseng roots, promotes wound repair, whereas glucocorticoids can inhibit the repair of airway epithelial damage in asthma.Objective: This study investigated whether PNS-R1 counteracts the inhibitory effects of glucocorticoids on the repair of airway epithelial damage in asthma. Methods: In vivo, female C57BL/6 mice were sensitized, challenged with house dust mites (HDM), and treated with dexamethasone, PNS-R1, and/or adenovirus GR beta-shRNA. Airway epithelium damage was examined using pathological sections of the trachea and bronchi, markers of airway inflammation, epithelial cells in bronchoalveolar lavage fluid, and expression of the E-cadherin protein. In vitro, we treated 16HBE cells with dexamethasone, PNS-R1, and/or GR beta-siRNA and detected cell proliferation and migration. The expression of GR beta and key components of MKP-1 and Erk1/2 were detected by western blotting.Results: In vivo, PNS-R1 reduced airway inflammation, hyperresponsiveness, and mucus hypersecretion; the combination of PNS-R1 and dexamethasone promoted airway epithelial integrity and reduced cell detachment. In vitro, PNS-R1 alleviated the inhibition of bronchial epithelial cell growth, migration, and proliferation by dexamethasone; PNS-R1 promoted GR beta expression, inhibited MKP-1 protein expression, and activated MAPK signaling, thereby promoting airway epithelial cell proliferation and repair. Conclusions: Panax notoginseng saponin R1 alleviated the inhibitory effect of dexamethasone on the repair of airway epithelial damage in asthmatic mice, likely by promoting the proliferation of airway epithelial cells by stimulating GR beta expression and activating the MAPK pathway."

基金机构：Youth Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders [YBRP-202129]; National Center for Clinical Medical Research in Children's Health and Diseases Clinical Medical Research Youth Project [NCRCCHD-2021-YP-0X-11]; Postdoctoral Science Foundation of Chongqing Natural Science Foundation [CSTB2022NSCQ-BHX0639]; Chongqing Natural Science Foundation General Project [CSTB2022NSCQ-MSX0871]

基金资助正文：This research was supported by grants from the Youth Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders (No.YBRP-202129) ; The National Center for Clinical Medical Research in Children's Health and Diseases Clinical Medical Research Youth Project (No.NCRCCHD-2021-YP-0X-11) ; The Postdoctoral Science Foundation of Chongqing Natural Science Foundation (No.CSTB2022NSCQ-BHX0639) ; The Chongqing Natural Science Foundation General Project (No.CSTB2022NSCQ-MSX0871) .