RNA Binding Protein PTBP1 Promotes the Metastasis of Gastric Cancer by Stabilizing PGK1 mRNA

作者全名："Wang, Xiaolin; Liang, Ce; Wang, Shimin; Ma, Qiang; Pan, Xiaojuan; Ran, Ai; Qin, Changhong; Huang, Bo; Yang, Feifei; Liu, Yuying; Zhang, Yuying; Ren, Junwu; Ning, Hao; Li, Haiping; Jiang, Yan; Xiao, Bin"

作者地址："[Wang, Xiaolin; Liang, Ce; Wang, Shimin; Ma, Qiang; Pan, Xiaojuan; Ran, Ai; Qin, Changhong; Huang, Bo; Yang, Feifei; Liu, Yuying; Zhang, Yuying; Ren, Junwu; Ning, Hao; Li, Haiping; Jiang, Yan; Xiao, Bin] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Huang, Bo] Zunyi Med Univ, Key Lab Basic Pharmacol Minist Educ & Joint Int Re, Zunyi 563006, Peoples R China"

通信作者："Xiao, B (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China."

来源：CELLS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001151881300001

JCR分区：Q2

影响因子：6

年份：2024

卷号：13

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：PTBP1; gastric cancer; PGK1; metastasis

摘要："Gastric cancer (GC) is the most common type of malignant tumor within the gastrointestinal tract, and GC metastasis is associated with poor prognosis. Polypyrimidine tract binding protein 1 (PTBP1) is an RNA-binding protein implicated in various types of tumor development and metastasis. However, the role of PTBP1 in GC metastasis remains elusive. In this study, we verified that PTBP1 was upregulated in GC tissues and cell lines, and higher PTBP1 level was associated with poorer prognosis. It was shown that PTBP1 knockdown in vitro inhibited GC cell migration, whereas PTBP1 overexpression promoted the migration of GC cells. In vivo, the knockdown of PTBP1 notably reduced both the size and occurrence of metastatic nodules in a nude mice liver metastasis model. We identified phosphoglycerate kinase 1 (PGK1) as a downstream target of PTBP1 and found that PTBP1 increased the stability of PGK1 by directly binding to its mRNA. Furthermore, the PGK1/SNAIL axis could be required for PTBP1's function in the promotion of GC cell migration. These discoveries suggest that PTBP1 could be a promising therapeutic target for GC."

基金机构：National Natural Science Foundation of China

基金资助正文：No Statement Available