Exploration and validation of signature genes and immune associations in septic cardiomyopathy

作者全名："Zhao, Zhengbo; Wang, Xiaojing; Tan, Fangyan; Liu, Han; Chen, Wan; Wang, Jing; Deng, Songbai; Du, Jianlin"

作者地址："[Zhao, Zhengbo] Yubei Dist Hosp TCM, Dept Cardiol, Chongqing, Peoples R China; [Wang, Xiaojing; Liu, Han; Chen, Wan] Jiulongpo Dist Peoples Hosp, Dept Cardiol, Chongqing, Peoples R China; [Tan, Fangyan; Wang, Jing; Deng, Songbai; Du, Jianlin] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Deng, SB; Du, JL (通讯作者)，Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, Chongqing, Peoples R China."

来源：CLINICAL AND EXPERIMENTAL HYPERTENSION

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001153169500001

JCR分区：Q3

影响因子：2.6

年份：2024

卷号：46

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Septic cardiomyopathy; weighted gene co-expression network analysis; immune infiltration analysis

摘要："An early and accurate diagnosis of septic cardiomyopathy is vital for improving the overall prognosis of sepsis. In our research, we aimed to identify signature genes and their immune connections in septic cardiomyopathy. By analyzing the mouse myocardial transcriptome from sepsis induced by cecum ligation and puncture (CLP), we identified four distinct k-means clusters. Further analysis of human myocardial datasets using Weighted Gene Co-expression Network Analysis (WGCNA) revealed a strong correlation between the MEturquoise module and septic cardiomyopathy (cor = 0.79, p < .001). Through the application of Cytoscape plug-in MCODE and comprehensive analysis, we pinpointed two signature genes, THBS1 and TIMP1. These genes demonstrated significant involvement in immune cell infiltration, as detected by CIBERSORT, and displayed promising prognostic potential as validated by external datasets. Our experimental validation confirmed the up-regulation of both THBS1 and TIMP1 in septic murine hearts, underscoring their positive association with septic cardiomyopathy."

基金机构：Natural Science Foundation of Chongqing Science and Technology Commission [cstc2020jcyj-msxmX0919]; CQMU Program for Youth Innovation in Future Medicine [W0133]; Senior Medical Talents Program of Chongqing for Young and Middle-Aged; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University

基金资助正文："This work was supported by the Natural Science Foundation of Chongqing Science and Technology Commission under Grant [cstc2020jcyj-msxmX0919], CQMU Program for Youth Innovation in Future Medicine [W0133], the Senior Medical Talents Program of Chongqing for Young and Middle-Aged, and the Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University."