Zipper-Confined DNA Nanoframe for High-Efficient and High-Contrast Imaging of Heterogeneous Tumor Cell

作者全名："Zhang, Mengxuan; Yang, Tiantian; Hu, Ruiwei; Li, Menghan; Liu, Yuanjie; He, Wen; Zhao, Lina; Xu, Yuan; Guo, Minghui; Ding, Shijia; Chen, Junman; Cheng, Wei"

作者地址："[Zhang, Mengxuan; Hu, Ruiwei; Li, Menghan; Liu, Yuanjie; He, Wen; Zhao, Lina; Xu, Yuan; Guo, Minghui; Ding, Shijia; Chen, Junman] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Zhang, Mengxuan; Yang, Tiantian; Cheng, Wei] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China; [Yang, Tiantian; Cheng, Wei] Chongqing Med Univ, Affiliated Hosp 1, Biobank Ctr, Chongqing 400016, Peoples R China"

通信作者："Chen, JM (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China.; Cheng, W (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China.; Cheng, W (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Biobank Ctr, Chongqing 400016, Peoples R China."

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:001158554000001

JCR分区：Q1

影响因子：7.4

年份：2024

卷号：96

期号：5

开始页：2253

结束页：2263

文献类型：Article

关键词：　

摘要："Current study in the heterogeneity and physiological behavior of tumor cells is limited by the fluorescence in situ hybridization technology in terms of probe assembly efficiency, background suppression capability, and target compatibility. In a typically well-designed assay, hybridization probes are constructed in a confined nanostructure to achieve a rapid assembly for efficient signal response, while the excessively high local concentration between different probes inevitably leads to nonspecific background leakage. Inspired by the fabric zipper, we propose a novel confinement reaction pattern in a zipper-confined DNA nanoframe (ZCDN), where two kinds of hairpin probes are independently anchored respective tracks. The metastable states of the dual tracks can well avoid signal leakage caused by the nonspecific probe configuration change. Biomarker-mediated proximity ligation reduces the local distance of dual tracks, kinetically triggering an efficient allosteric chain reaction between the hairpin probes. This method circumvents nonspecific background leakage while maintaining a high efficiency in responding to targets. ZCDN is employed to track different cancer biomarkers located in both the cytoplasm and cytomembrane, of which the expression level and oligomerization behavior can provide crucial information regarding intratumoral heterogeneity. ZCDN exhibits high target response efficiency and strong background suppression capabilities and is compatible with various types of biological targets, thus providing a desirable tool for advanced molecular diagnostics."

基金机构："Chongqing Medical University [82302621, 82272432, 82372334, 81873972]; National Natural Science Foundation of China [cstc2021jcyj-bsh X0153]; Natural Science Foundation of Chongqing [Postdoctoral Foundation] [CQYC20210302385]; Chongqing Talents-Innovation Leader Project [KJQN202300435]; Science and Technology Research Program of Chongqing Municipal Education Commission [W0183]; Future Medical Youth Innovation Team Development Support Program Project of Chongqing Medical University [JYPY202204]; Scientific Training Foundation of Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education) of College of Laboratory Medicine [BJRC202226]; Chongqing Medical University Graduate Talent Training Program"

基金资助正文："This study was supported by National Natural Science Foundation of China [82302621, 82272432, 82372334, and 81873972], Natural Science Foundation of Chongqing [Postdoctoral Foundation, grant no. cstc2021jcyj-bsh X0153], the Chongqing Talents-Innovation Leader Project [CQYC20210302385], Science and Technology Research Program of Chongqing Municipal Education Commission [grant no. KJQN202300435], Future Medical Youth Innovation Team Development Support Program Project of Chongqing Medical University [grant no. W0183], Scientific Training Foundation of Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education) of College of Laboratory Medicine [grant no. JYPY202204], and Chongqing Medical University Graduate Talent Training Program [grant no. BJRC202226]."