TonEBP: A Key Transcription Factor in Microglia Following Intracerebral Hemorrhage Induced-Neuroinflammation

作者全名："Palahati, Ailiyaer; Luo, Yujia; Qin, Le; Duan, Yuhao; Zhang, Mi; Gan, Hui; Zhai, Xuan"

作者地址："[Palahati, Ailiyaer; Luo, Yujia; Qin, Le; Duan, Yuhao; Zhang, Mi; Gan, Hui; Zhai, Xuan] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Neurosurg, Minist Educ,Key Lab Child Dev & Disorders,Children, Chongqing 400010, Peoples R China; [Palahati, Ailiyaer; Luo, Yujia; Qin, Le; Duan, Yuhao; Zhang, Mi; Gan, Hui; Zhai, Xuan] Chongqing Med Univ, Ctr Neurosci Res, Chongqing 400016, Peoples R China"

通信作者："Gan, H; Zhai, X (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Neurosurg, Minist Educ,Key Lab Child Dev & Disorders,Children, Chongqing 400010, Peoples R China.; Gan, H; Zhai, X (通讯作者)，Chongqing Med Univ, Ctr Neurosci Res, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ESI学科分类：CHEMISTRY

WOS号：WOS:001161090600001

JCR分区：Q2

影响因子：5.6

年份：2024

卷号：25

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：intracerebral hemorrhage; inflammation; TonEBP; NF-kappa B; PELI1

摘要："Transcription factors within microglia contribute to the inflammatory response following intracerebral hemorrhage (ICH). Therefore, we employed bioinformatics screening to identify the potential transcription factor tonicity-responsive enhancer-binding protein (TonEBP) within microglia. Inflammatory stimuli can provoke an elevated expression of TonEBP in microglia. Nevertheless, the expression and function of microglial TonEBP in ICH-induced neuroinflammation remain ambiguous. In our recent research, we discovered that ICH instigated an increased TonEBP in microglia in both human and mouse peri-hematoma brain tissues. Furthermore, our results indicated that TonEBP knockdown mitigates lipopolysaccharide (LPS)-induced inflammation and the activation of NF-kappa B signaling in microglia. In order to more deeply comprehend the underlying molecular mechanisms of how TonEBP modulates the inflammatory response, we sequenced the transcriptomes of TonEBP-deficient cells and sought potential downstream target genes of TonEBP, such as Pellino-1 (PELI1). PELI has been previously reported to mediate nuclear factor-kappa B (NF-kappa B) signaling. Through the utilization of CUT & RUN, a dual-luciferase reporter, and qPCR, we confirmed that TonEBP is the transcription factor of Peli1, binding to the Peli1 promoter. In summary, TonEBP may enhance the LPS-induced inflammation and activation of NF-kappa B signaling via PELI1."

基金机构：National Natural Science Foundation of China

基金资助正文：No Statement Available