Proteomic profiling of aqueous humor-derived exosomes in Vogt-Koyanagi-Harada disease and Behcet's uveitis
作者全名："Zhang, Yinan; Deng, Yang; Jing, Shixiang; Su, Guannan; Li, Na; Huang, Ziqian; Zhang, Wanyun; Chen, Zhijun; Yang, Peizeng"
作者地址："[Zhang, Yinan; Jing, Shixiang; Li, Na; Yang, Peizeng] Zhengzhou Univ, Henan Prov Eye Hosp, Dept Ophthalmol,Afliated Hosp 1, Henan Int Joint Res Lab Ocular Immunol & Retinal I, Zhengzhou, Henan, Peoples R China; [Zhang, Yinan; Jing, Shixiang] Zhengzhou Univ, Acad Med Sci, Zhengzhou, Henan, Peoples R China; [Deng, Yang; Su, Guannan; Huang, Ziqian; Zhang, Wanyun; Chen, Zhijun; Yang, Peizeng] Chongqing Med Univ, Chongqing Eye Inst, Chongqing Branch,Municipal Div,Natl Clin Res Ctr O, Chongqing Key Lab Ophthalmol,Affiliated Hosp 1, Chongqing, Peoples R China; [Yang, Peizeng] Zhengzhou Univ, Henan Prov Eye Hosp, Dept Ophthalmol,Affiliated Hosp 1, Henan Int Joint Res Lab Ocular Immunol & Retinal I, Jianshe East Rd 1, Zhengzhou 450052, Henan, Peoples R China"
通信作者："Yang, PZ (通讯作者)，Zhengzhou Univ, Henan Prov Eye Hosp, Dept Ophthalmol,Affiliated Hosp 1, Henan Int Joint Res Lab Ocular Immunol & Retinal I, Jianshe East Rd 1, Zhengzhou 450052, Henan, Peoples R China."
来源：CLINICAL IMMUNOLOGY
ESI学科分类：IMMUNOLOGY
WOS号：WOS:001161917200001
JCR分区：Q1
影响因子：8.6
年份：2024
卷号：259
期号：
开始页：
结束页：
文献类型：Article
关键词：Vogt-Koyanagi-Harada disease; Behcet 's uveitis; Aqueous humor; Exosome; Proteomics
摘要："Vogt-Koyanagi-Harada (VKH) disease and Behcet's uveitis (BU) are the two major vision-threatening uveitis entities. This study performed the first label-free quantitative proteomics on aqueous humor-derived exosomes from 84 patients with VKH or BU to determine their potential roles. Sixty-five differentially expressed proteins (DEPs) and 40 DEPs were detected in the VKH and BU groups, respectively. GO and KEGG analysis showed that DEPs were mainly enriched in the complement-related pathways. The complement C1q subcomponent subunit B (C1QB) was identified as a key exosomal protein, and its expression was significantly increased by western blotting in both diseases. Additionally, the integrated analysis based on the published scRNA-seq data showed that C1QB-containing exosomes were mainly produced by mononuclear macrophages in the anterior segment tissue. Overall, our proteomic profiling highlights that complement-related pathways may be actively involved in the pathogenesis of these two diseases. These pathways may also serve as treatment targets for both diseases."
基金机构：Major Program of Medical Science and Technology Project of Health Commission of Henan Province [SBGJ202101011]; Talent Development Program for the Medical College of Zhengzhou University; National Natural Science Foundation Key Program [82230032]; National Natural Science Foundation Project [82101108]; Medical Scientific and Technological Project of Henan Province [SBGJ2020003031]
基金资助正文："This study was supported by the Major Program of Medical Science and Technology Project of Health Commission of Henan Province (SBGJ202101011) , the Talent Development Program for the Medical College of Zhengzhou University, the National Natural Science Foundation Key Program (82230032) , the National Natural Science Foundation Project (82101108) , and the Medical Scientific and Technological Project of Henan Province (SBGJ2020003031) ."
