Characterization of T cell receptor repertoire in penile cancer

作者全名："Zhang, Junying; Wang, Yapeng; Huang, Yiqiang; Tan, Xintao; Xu, Jing; Yan, Qian; Tan, Jiao; Zhang, Yao; Zhang, Jun; Ma, Qiang; Zhu, Hailin; Ye, Jin; Zhu, Zhaojing; Lan, Weihua"

作者地址："[Zhang, Junying; Zhu, Zhaojing] Chongqing Med & Pharmaceut Coll, Chongqing Engn Res Ctr Pharmaceut Sci, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing 401331, Peoples R China; [Zhang, Junying] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Wang, Yapeng; Huang, Yiqiang; Tan, Xintao; Xu, Jing; Yan, Qian; Zhang, Yao; Zhang, Jun; Ma, Qiang; Zhu, Hailin; Lan, Weihua] Army Med Univ, Daping Hosp, Dept Urol, Chongqing 400042, Peoples R China; [Ye, Jin] Thirteenth Peoples Hosp Chongqing, Urinary Nephropathy Ctr, Chongqing 400053, Peoples R China; [Tan, Jiao] Chongqing Med & Pharmaceut Coll, Sch Pharm, Chongqing 401331, Peoples R China"

通信作者："Zhu, ZJ (通讯作者)，Chongqing Med & Pharmaceut Coll, Chongqing Engn Res Ctr Pharmaceut Sci, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing 401331, Peoples R China.; Lan, WH (通讯作者)，Army Med Univ, Daping Hosp, Dept Urol, Chongqing 400042, Peoples R China.; Ye, J (通讯作者)，Thirteenth Peoples Hosp Chongqing, Urinary Nephropathy Ctr, Chongqing 400053, Peoples R China."

来源：CANCER IMMUNOLOGY IMMUNOTHERAPY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001164585000009

JCR分区：Q1

影响因子：5.8

年份：2024

卷号：73

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：Penile cancer; Tumor microenvironment; High-throughput sequencing; TCR repertoire; TCR clonality

摘要："Tumor-infiltrating lymphocytes (TILs) play a key role in regulating the host immune response and shaping tumor microenvironment. It has been previously shown that T cell infiltration in penile tumors was associated with clinical outcomes. However, few studies have reported the T cell receptor (TCR) repertoire in patients with penile cancer. In the present study, we evaluated the TCR repertoires in tumor and adjacent normal tissues from 22 patients with penile squamous cell carcinoma (PSCC). Analysis of the T cell receptor beta-variable (TRBV) and joining (TRBJ) genes usage and analysis of complementarity determining region 3 (CDR3) length distribution did not show significant differences between tumor and matched normal tissues. Moreover, analysis of the median Jaccard index indicated a limited overlap of TCR repertoire between these groups. Compared with normal tissues, a significantly lower diversity and higher clonality of TCR repertoire was observed in tumor samples, which was associated with clinical characteristics. Further analysis of transcriptional profiles demonstrated that tumor samples with high clonality showed increased expression of genes associated with CD8 + T cells. In addition, we analyzed the TCR repertoire of CD4 + T cells and CD8 + T cells isolated from tumor tissues. We identified that expanded clonotypes were predominantly in the CD8 + T cell compartment, which presented with an exhausted phenotype. Overall, we comprehensively compared TCR repertoire between penile tumor and normal tissues and demonstrated the presence of distinct T cell immune microenvironments in patients with PSCC."

基金机构：Science and technology research program of Chongqing Education Commission of China [KJQN202302805]; Natural science foundation of Chongqing [CSTB2023NSCQ-BHX0225]; School-level project fund of Chongqing Medical and Pharmaceutical College [ygz2022102]

基金资助正文："The authors thank all patients in this study for their collaboration. This work was supported by grants from the science and technology research program of Chongqing Education Commission of China (No. KJQN202302805), the natural science foundation of Chongqing (No. CSTB2023NSCQ-BHX0225), and the school-level project fund of Chongqing Medical and Pharmaceutical College (No. ygz2022102)."