Reinforced dentin remineralization via a novel dual-affinity peptide

作者全名："Fan, Menglin; Zhou, Zilin; Zhu, Wanchun; Li, Meng; Tu, Yuanyuan; Yu, Zhaohan; Li, Jiyao; Zhang, Min; Liang, Kunneng"

作者地址："[Fan, Menglin; Zhou, Zilin; Li, Meng; Tu, Yuanyuan; Yu, Zhaohan; Li, Jiyao; Zhang, Min; Liang, Kunneng] Sichuan Univ, State Key Lab Oral Dis, Natl Clin Res Ctr Oral Dis, Dept Cariol & Endodont,West China Hosp Stomatol, Chengdu 610041, Peoples R China; [Zhu, Wanchun] Chongqing Med Univ, Stomatol Coll, Chongqing 401147, Peoples R China"

通信作者："Zhang, M; Liang, KN (通讯作者)，Sichuan Univ, State Key Lab Oral Dis, Natl Clin Res Ctr Oral Dis, Dept Cariol & Endodont,West China Hosp Stomatol, Chengdu 610041, Peoples R China."

来源：DENTAL MATERIALS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001169554700001

JCR分区：Q1

影响因子：5

年份：2024

卷号：40

期号：2

开始页：254

结束页：266

文献类型：Article

关键词：Dentin; Collagen; Hydroxyapatite; Adsorption; Remineralization; Caries

摘要："Objectives: In light of the constantly flowing saliva, anti-caries remineralization agents are inclined to be taken away. Owing to their limited residence time, the remineralization effect is not as desirable as expected. Hence, our study aimed to synthesize a novel peptide (DGP) with high affinity to both collagen fibrils and hydroxyapatite, and investigated its dentin remineralization efficacy in vitro and anti-caries capability in vivo. Methods: DGP was synthesized through Fmoc solid-phase reaction. The binding ability and interaction mechanism of DGP to demineralized dentin were investigated. Dentin specimens were demineralized, then treated with DGP and deionized water respectively. The specimens were incubated in artificial saliva and in-vitro remineralization effectiveness was analyzed after 14 days. The rat caries model was established to further scrutinize the in-vivo efficacy of caries prevention. Results: DGP possesses an enhanced adhesion force of 12.29 +/- 1.12 nN to demineralized dentin. The favorable adsorption capacity is ascribed to the stable hydrogen bonds between S2P-101 and ASP-100 of DGP and GLY33 and PRO-16 of collagen fibers. Abundant mineral deposits and remarkable tubule occlusion were observed in the DGP group. DGP-treated dentin obtained notable microhardness recovery and higher mineral content after a 14day remineralization regimen. DGP also demonstrated potent caries prevention in vivo, with substantially fewer carious lesions and significantly lower Keyes scoring. Significance: DGP proves to possess a high affinity to demineralized dentin regardless of saliva flowing, thus enhancing remineralization potency significantly in vitro and in vivo, potential for dental caries prevention and combatting initial dentin caries clinically."

基金机构："National Natural Science Foundation of China [82270970, 82170949, 81991500, 81991501]; Key Research and Development Program of Sichuan Province [2022YFS0288, 2023YFS0048]; Chengdu Science and Technology Support Program [2021-YF05-02061-SN, 0040304153013]; China Postdoctoral Foundation [2018M643507]"

基金资助正文："This work was supported by National Natural Science Foundation of China (82270970, 82170949, 81991500 and 81991501) ; Key Research and Development Program of Sichuan Province (2022YFS0288 and 2023YFS0048) ; Chengdu Science and Technology Support Program (2021-YF05-02061-SN) ; Postdoctoral Cross Funding of Sichuan Uni- versity (0040304153013) ; China Postdoctoral Foundation 2018M643507 (L.K.N) ."