<i>Escherichia coli</i> and HPV16 coinfection may contribute to the development of cervical cancer

作者全名："Zou, Qin; Wu, Yingying; Zhang, Shuaishuai; Li, Shu; Li, Siyue; Su, Yan; Zhang, Lei; Li, Qian; Zou, Hua; Zhang, Xinyuan; Wang, Teng; Liang, Shuang; Yang, Jun; Li, Chunli"

作者地址："[Zou, Qin; Wu, Yingying; Li, Shu; Li, Siyue; Su, Yan; Zhang, Lei; Li, Qian; Zou, Hua; Zhang, Xinyuan; Li, Chunli] Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Clin Lab, Women & Childrens Hosp, Chongqing, Peoples R China; [Zhang, Shuaishuai] Chongqing Univ, Dept Clin Lab, Three Gorges Hosp, Chongqing, Peoples R China; [Wang, Teng] Chongqing Med Univ, Dept Hematol, Affiliated Hosp 2, Chongqing, Peoples R China; [Liang, Shuang] Chongqing Med Univ, Women & Childrens Hosp, Chongqing Hlth Ctr Women & Children, Dept Pathol, Chongqing, Peoples R China; [Yang, Jun] Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Obstet & Gynecol, Women & Childrens Hosp, Chongqing, Peoples R China"

通信作者："Li, CL (通讯作者)，Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Clin Lab, Women & Childrens Hosp, Chongqing, Peoples R China."

来源：VIRULENCE

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001173603000001

JCR分区：Q2

影响因子：5.2

年份：2024

卷号：15

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Cervical cancer; precursors; HPV; E. coli; co-infection

摘要："Persistent human papillomavirus HPV infection is a necessary but insufficient condition for cervical cancer. Microorganisms are crucial environmental factors in cancers susceptibility and progression, recently attracting considerable attention. This study aimed to determine the infection status and relationship between high-risk HPV (HR-HPV) and lower genital tract infectious pathogens in cervical cancer and its precursors. From a retrospective and a prospective cohort analysis, Escherichia coli (E. coli) dominated the pathogens isolated from cervical discharges, and an isolation rate uptrend has been shown recently. HPV16 and E. coli's coinfection rate gradually increased with the severity of cervical intraepithelial neoplasia. The adhesion and invasion abilities of the isolated E. coli to HPV16-positive SiHa cells were evaluated in vitro. The TCGA database and cervical tissues samples analysis showed that IL-10 was upregulated in cervical cancer. IL-10 expression levels increased in tissue samples with the severity of cervical cancer and its precursors with HPV16 and E. coli coinfection. Although no significant changes in IL-10 production were observed in the co-culture supernatant, we hypothesized that Treg immune cells in the tumour microenvironment might be responsible for the local IL-10 upregulation, according to our data showing Foxp3 upregulation and an upward trend with the cervical intraepithelial neoplasia grading to cancer and tumours with E. coli and HPV16 coinfection. Our data provide insights into the possible role of E. coli in cervical cancer progression and suggest that the application of HPV and E. coli screening programs may be an effective strategy to relieve the burden of cervical cancer and its precursor lesions."

基金机构："Natural Science Foundation of Chongqing, China [cstc2020jcyj-msxmX0588, CSTB2022NSCQ-MSX0807]"

基金资助正文："This work was Sponsored by Natural Science Foundation of Chongqing, China [grant no. cstc2020jcyj-msxmX0588, CSTB2022NSCQ-MSX0807]."