Primary study on the effects and mechanisms of separate and combined decoctions of Scutellaria baicalensis Georgi - Coptis chinensis Franch extracts in relieving acute alcoholic liver injury in rats

作者全名："Shen, Yingyan; Zhao, Yixin; Zhong, Chunxue; Huang, Hanmei; Yang, Zaiqing; Wu, Mingxing; Lu, Lingzhi; Yang, Rongping; Ke, Xiumei"

作者地址："[Shen, Yingyan; Zhao, Yixin] Chengdu Univ Tradit Chinese Med, Key Lab Breeding Base Systemat Res & Utilizat Chin, Med Resources Cofounded Sichuan Prov & Minist Sci, Chengdu, Peoples R China; [Zhong, Chunxue; Huang, Hanmei; Yang, Zaiqing; Wu, Mingxing; Lu, Lingzhi; Yang, Rongping] Southwest Univ, Chongqing Key Lab Chinese Med New Drug Screening, Chongqing, Peoples R China; [Ke, Xiumei] Chongqing Med Univ, Coll Pharm, Chongqing, Peoples R China; [Yang, Rongping] Southwest Univ, 2 Tiansheng Rd, Chongqing 400715, Peoples R China; [Ke, Xiumei] Chongqing Med Univ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："Yang, RP (通讯作者)，Southwest Univ, 2 Tiansheng Rd, Chongqing 400715, Peoples R China.; Ke, XM (通讯作者)，Chongqing Med Univ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：JOURNAL OF ETHNOPHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001177846200001

JCR分区：Q1

影响因子：5.4

年份：2024

卷号：324

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Scutellaria baicalensis Georgi; Coptis chinensis Franch; Separate decoctions; Combined decoctions; Acute alcoholic liver injury; Network pharmacology

摘要："Ethnopharmacological relevance: Scutellaria baicalensis Georgi (SBG) and Coptis chinensis Franch (CCF) are traditional herbal medicine pairs used for clearing heat and eliminating dampness, stopping diarrhea, and detoxification. Traditionally, these two herbs are combined and decocted together, but the modern preparation procedures separate them to avoid the large amount of precipitation generated from co-decoction. Thus, a conflict lies between the traditional and modern extraction processes of Scutellaria baicalensis Georgi - Coptis chinensis Franch (SBG-CCF). Aim of study: There is a conflict between traditional medical practices of SBG-CCF and the modern formulation industry. In this study, we investigated the differences in the effects and mechanisms of SBG-CCF extracted by decocting separately and combining decoctions, as well as the scientific effectiveness of traditional and modern treatment methods on both. Acute alcoholic liver injury (ALI) rats were used as the pathological model. Materials and methods: SD rats were divided into 8 groups, including blank group, model group, low, medium, and high dose groups of SBG-CCF separated decoction, low, medium, and high dose groups of SBG-CCF combined decoction. Acute alcoholic liver injury model was induced in rats by gradually increasing the dose of alcohol through gavage everyday using white wine with an alcohol content 52%. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), triglyceride (TG), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH) were used as indicators to assess the intervention effect of SBG-CCF. And the potential active ingredients of SBG-CCF and the targets related to ALI were screened using network pharmacology, and the prediction results of network pharmacology were verified by quantitative real-time fluorescence PCR (qRT-PCR). Results: SBG-CCF decoction alone and six combinations of decoctions have different degrees of improvement on alcoholic liver injury, with significant efficacy in the middle-dose group, and the combined decoction was superior to the individual decoction. SBG-CCF gavage can reduce the activity of AST, ALT, TC, TG, LDH, and MDA in the serum and liver of ALI rats, while increasing the levels of SOD and GSH. Network pharmacological analysis identified 39 active components, mainly flavonoids and alkaloids. Enrichment analysis suggested that SBG-CCF may treat ALI through the regulation of tumor necrosis factor (TNF), mitogen-activated protein kinase (MAPK), interleukin-17 (IL-17), apoptosis, and the Toll-like receptor signaling pathways. The key targets in the DiseaseSignaling Pathway-Target Network were MAPK8, IKBKB, MAPK10, MAPK3, MAPK1, and AKT1. qRT-PCR results indicated that targets regulating inflammation and lipid metabolism are MAPK8, MAPK10, MAPK3, and AKT1. Conclusion: SBG-CCF separately extracts and combines decoction can alleviate acute alcoholic liver injury, and the effect of combined decoction is more significant than separate decoction, implying that the precipitate produced by the combination of the two is also an active substance. The resistance mechanism of SBG-CCF ALI"

基金机构：National Natural Science Foundation of China [81960721]; Xinglin program of Chongqing TCM/TCM-integrated Key discipline [2021-ZDXK-yc11]

基金资助正文：This work was supported by the National Natural Science Foundation of China (NO. 81960721) ; Xinglin program of Chongqing TCM/TCM-integrated Key discipline (2021-ZDXK-yc11) .