Assessment of Reference Genes Stability in Cortical Bone of Obese and Diabetic Mice

作者全名："Ai, Yuanli; Peng, Kun; Li, Chunli; Zhang, Jun; Wang, Gang; Wang, Bin; Huang, Enyi"

作者地址："[Ai, Yuanli; Li, Chunli; Zhang, Jun; Wang, Bin] Chongqing Med Univ, Inst Life Sci, Coll Basic Med, Chongqing, Peoples R China; [Peng, Kun] Chongqing Med & Pharmaceut Coll, Chongqing, Peoples R China; [Wang, Gang; Huang, Enyi] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing, Peoples R China; [Wang, Gang; Huang, Enyi] Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Coll Stomatol, Chongqing, Peoples R China"

通信作者："Wang, B (通讯作者)，Chongqing Med Univ, Inst Life Sci, Coll Basic Med, Chongqing, Peoples R China.; Huang, EY (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing, Peoples R China.; Huang, EY (通讯作者)，Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Coll Stomatol, Chongqing, Peoples R China."

来源：DIABETES METABOLIC SYNDROME AND OBESITY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001178766700001

JCR分区：　

影响因子：　

年份：2024

卷号：17

期号：　

开始页：1081

结束页：1091

文献类型：Article

关键词：reference genes; Rpl13a; Gapdh; cortical bone; metabolic disorders

摘要："Introduction: Bone, a pivotal structural organ, is susceptible to disorders with profound health implications. The investigation of gene expression in bone tissue is imperative, particularly within the context of metabolic diseases such as obesity and diabetes that augment the susceptibility to bone fractures. The objective of this study is to identify a set of internal control genes for the analysis of gene expression. Methods: This study employs reverse transcription-quantitative real -time polymerase chain reaction (RT-qPCR) to assess gene expression in bone tissue. We selected fourteen housekeeping genes and assessed their stability in the cortical bone of mouse models for obesity and diabetes using four well-established algorithms (GeNorm, BestKeeper, NormFinder, and the comparative Delta Ct method). Results and Conclusion: We identified Rpl13a as the mostly stably expressed reference gene in cortical bone tissue from mouse models of obesity and diabetes (db/db), while Gapdh was found to be the most stable reference gene in another diabetes model, KKAy mice. Additionally, Ef1a, Ppia, Rplp0, and Rpl22 were identified as alternative genes suitable for normalizing gene expression in cortical bone from obesity and diabetes mouse models. These findings enhance RT-qPCR accuracy and reliability, offering a strategic guide to select reference gene for studying bone tissue gene expression in metabolic disorders."

基金机构："Natural Science Foundation of Chongqing (China) [CSTB2022NSCQ-MSX0110, cstc2021jcyj-msxmX0320]; Future Medical Youth Innovation Program of Chongqing Medical University [W0046]; Chongqing Medical University"

基金资助正文："Acknowledgment This research was funded by Natural Science Foundation of Chongqing (China) , CSTB2022NSCQ-MSX0110, cstc2021jcyj-msxmX0320; the Future Medical Youth Innovation Program of Chongqing Medical University, W0046. The APC was funded by Chongqing Medical University."