Multifaceted SOX2-chromatin interaction underpins pluripotency progression in early embryos

作者全名："Li, Lijia; Lai, Fangnong; Hu, Xiaoyu; Liu, Bofeng; Lu, Xukun; Lin, Zili; Liu, Ling; Xiang, Yunlong; Frum, Tristan; Halbisen, Michael A.; Chen, Fengling; Fan, Qiang; Ralston, Amy; Xie, Wei"

作者地址："[Li, Lijia; Lai, Fangnong; Hu, Xiaoyu; Liu, Bofeng; Lu, Xukun; Liu, Ling; Chen, Fengling; Fan, Qiang; Xie, Wei] Tsinghua Univ, Ctr Stem Cell Biol & Regenerat Med, Sch Life Sci, MOE Key Lab Bioinformat,New Cornerstone Sci Lab, Beijing 100084, Peoples R China; [Li, Lijia; Lai, Fangnong; Hu, Xiaoyu; Liu, Bofeng; Lu, Xukun; Liu, Ling; Chen, Fengling; Fan, Qiang; Xie, Wei] Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China; [Lin, Zili] Beijing Univ Agr, Coll Anim Sci, Beijing 102206, Peoples R China; [Lin, Zili] Beijing Univ Agr, Technol Coll, Beijing 102206, Peoples R China; [Xiang, Yunlong] Chongqing Med Univ, Sch Basic Med Sci, Dept Cell Biol & Genet, Chongqing 400016, Peoples R China; [Frum, Tristan; Halbisen, Michael A.; Ralston, Amy] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA; [Frum, Tristan] Univ Michigan, Med Sch, Dept Internal Med, Gastroenterol, Ann Arbor, MI 48109 USA"

通信作者："Xie, W (通讯作者)，Tsinghua Univ, Ctr Stem Cell Biol & Regenerat Med, Sch Life Sci, MOE Key Lab Bioinformat,New Cornerstone Sci Lab, Beijing 100084, Peoples R China.; Xie, W (通讯作者)，Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China."

来源：SCIENCE

ESI学科分类：Multidisciplinary

WOS号：WOS:001183708700028

JCR分区：Q1

影响因子：56.9

年份：2023

卷号：382

期号：6676

开始页：　

结束页：　

文献类型：Article

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摘要："Pioneer transcription factors (TFs), such as OCT4 and SOX2, play crucial roles in pluripotency regulation. However, the master TF-governed pluripotency regulatory circuitry was largely inferred from cultured cells. In this work, we investigated SOX2 binding from embryonic day 3.5 (E3.5) to E7.5 in the mouse. In E3.5 inner cell mass (ICM), SOX2 regulates the ICM-trophectoderm program but is dispensable for opening global enhancers. Instead, SOX2 occupies preaccessible enhancers in part opened by early-stage expressing TFs TFAP2C and NR5A2. SOX2 then widely redistributes when cells adopt naive and formative pluripotency by opening enhancers or poising them for rapid future activation. Hence, multifaceted pioneer TF-enhancer interaction underpins pluripotency progression in embryos, including a distinctive state in E3.5 ICM that bridges totipotency and pluripotency."

基金机构："National Key R&D Program of China [2021YFA1100102, 2019YFA0508900]; National Natural Science Foundation of China [31988101, 31830047]; Tsinghua-Peking Center for Life Sciences; NIH [R35 GM131759, R01 HD108722]; HHMI International Research Scholar; New Cornerstone Investigator"

基金资助正文："This work was funded by the National Key R & D Program of China (2021YFA1100102 to W.X.); the National Natural Science Foundation of China (31988101 and 31830047 to W.X.); the National Key R & D Program of China (2019YFA0508900 to W.X.); and the Tsinghua-Peking Center for Life Sciences (to W.X.)M.A.H., T.F., and A.R. have been supported by NIH R35 GM131759 and R01 HD108722. W.X. is a recipient of an HHMI International Research Scholar and a New Cornerstone Investigator."